数字农科院2.0

Implication of a galactomannan-binding GH2 beta-mannosidase in mannan utilization by Caldicellulosiruptor bescii

文献类型： 外文期刊

作者： Liang, Di;Xie, Xiangming;Liang, Di;Gong, Li;Yao, Bin;Xu, Xianli;Qin, Xing;Ma, Rui;Luo, Huiying;Su, Xiaoyun

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关键词： Caldicellulosiruptor bescii;Glycoside hydrolase;Mannosidase;GH2;Consolidated bioprocessing

期刊名称： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

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收录情况： SCI

摘要： Many glycoside hydrolases involved in deconstruction of cellulose and xylan from the excellent plant cell wall polysaccharides-degrader Caldicellulosiruptor bescii have been cloned and analyzed. However, far less is known about the enzymatic breakdown of mannan, an important component of hemicellulose. We herein cloned, expressed and purified the first B-mannosidase CbMan2A from C. bescii. CbMan2A is thermophilic, with an optimal temperature of 80 degrees C. CbMan2A hydrolyzes mannooligosaccharides with degrees of polymerization from 2 to 6 mainly into mannose and shows strong synergy with CbMan5A, an endo-mannanase from the same bacterium, in releasing mannose from beta-1,4-mannan. Thus CbMan2A forms the missing link in enzymatic conversion of mannan into the ready-to-use mannose by C Based on these observations, a model illustrating how CbMan2A may assist C bescii in mannan utilization is presented. In addition, CbMan2A appeared to bind to insoluble galactomannan in a pH-dependent fashion. Although the relation of this feature to mannan utilization remains elusive, CbMan2A represents an excellent model for investigation of the binding of GH2 beta-mannosidases to galactomannan. (C) 2015 Elsevier Inc. All rights reserved.

分类号： Q5

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