数字农科院2.0

An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons

文献类型： 外文期刊

作者： Meng, X. Y.;Li, Y. S.;Zhou, Y.;Sun, Y.;Qiao, B.;Si, C. C.;Hu, P.;Lu, S. Y.;Ren, H. L.;Liu, Z. S.;Meng, X. Y.;Qiu, H. J.;Sun, Y.;Zhou, Y.;Liu, J. Q.

作者机构：

关键词： PAHs;Improved RT-IPCR;Detection

期刊名称： BIOSENSORS & BIOELECTRONICS

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年卷期：

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收录情况： SCI

摘要： Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous homogeneous chemicals which are well known by carcinogens, mutagens and endocrine disorder. Here, an improved real-time immuno-PCR (RT-IPCR) was developed for detection of pyrene and its homologs in water samples. The PAHs in sample compete with pyrene-modified DNA to bind with monoclonal antibody (McAb) coated on PCR plate. The reporter DNA was exponentially amplified by real-time PCR instrument using Fast Start universal SYBR Green Master (ROX) kit. Only two reaction steps were needed to accomplish the detection. The assay had a good linear range from 5 pmol L-1 to 5 nmol L-1 with a detection limit of 3.5 pmol L-1. For application assay, the average recoveries from tap water, lake water and mineral water were 98.4%, 98.2% and 99.7%, respectively which showed a good correlation (R-2=0.9906) with those from GC-MS. The results indicated that the improved RT-IPCR seems to be a potential method for simple and ultrasensitive detection of pyrene and some homologues in environment water samples. (C) 2015 Elsevier B.V. All rights reserved.

分类号： Q

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