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Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system

文献类型： 外文期刊

作者： Khan, Sehroon;Xu, Jianchu;Khan, Sehroon;Xu, Jianchu;Khan, Sehroon;Guo Lihua;Qiu Dewen;Nadir, Sadia;Nadir, Sadia;Holmes, Keith A.

作者机构：

关键词： Insect-toxic protein;Bb70p;Entomopathogenic fungi;Beauveria bassiana;Galleria mellonella;Filtrate

期刊名称： JOURNAL OF INVERTEBRATE PATHOLOGY

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年卷期：

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收录情况： SCI

摘要： An insect-toxic protein, Bb70p, was purified from Beauveria bassiana 70 using ammonium sulfate precipitation, ion exchange chromatography, and gel filtration. Bb70p has a high affinity for anion exchangers and 2D electrophoresis results revealed a single spot with a molecular weight of 35.5 kDa and an iso-electric point of similar to 4.5. Bb70p remains active from 4 to 60 degrees C, within a pH range of 4-10, but is more active in slightly acidic pH. A pure protein, Bb70p does not have any carbohydrate side chains. The protein caused high mortality by intra-haemocelic injection into Galleria mellonella with LD50 of 334.41 mu g/g body weight and activates the phenol oxidase cascade. With a partial amino acid sequence comparison using the NCBI database, we showed no homology to known toxin proteins of entomopathogenic fungi. Thus, Bb70p appears to be an insect toxin protein, demonstrating novelty. Identification of this insect-toxic protein presents potential to enhance the virulence of B. bassiana through genetic manipulation. (C) 2015 Elsevier Inc. All rights reserved.

分类号： Q959.1

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