数字农科院2.0

Cloning, Expression, Purification, and Insecticidal Activity of a Novel Cry1Na3 Toxin From Bacillus thuringiensis BRC-ZYR2

文献类型： 外文期刊

作者： Su, Xiaoyu;Jin, Xin;Guan, Xiong;Chen, Chen;Huang, Tianpei;Dai, Ruiqing;Su, Xiaoyu;Jin, Xin;Guan, Xiong;Chen, Chen;Huang, Tianpei;Dai, Ruiqing;Zhang, Jie;Shu, Changlong

作者机构：

关键词： Bacillus thuringiensis;Cry1Na3;Plutella xylostella (L.);Ostrinia furnacalis (Guenee)

期刊名称： JOURNAL OF ECONOMIC ENTOMOLOGY

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收录情况： SCI

摘要： Bacillus thuringiensis produces a variety of insecticidal crystal proteins (ICPs). Genome sequencing is a promising strategy for detecting and identifying B. thuringiensis ICPs, which are of great interest to the biocontrol field. In this study, a novel ICP gene was cloned from B. thuringiensis BRC-ZYR2 based on genomic data from 454 GS-FLX Titanium sequencing and an analysis of the results using the B. thuringiensis Toxin_Scanner (http://bcam.hzaubmb.net/BtToxin_scanner/index.php). cry1Na3 designated by the B. thuringiensis Toxin Nomenclature Committee, encoded a 601-amino acid, 68.0-kDa protein that exhibited 95% identity with Cry1Na1 and 99% identity with Cry1Na2. Cry1Na3 contained three conserved domains commonly found in three-domain ICPs. Cry1Na3 was toxic to Plutella xylostella (L.) and Ostrinia furnacalis (Guenee), with LC50 values of 3.69 mu g/ml and 31.30 mu g/ml, respectively. However, Laodelphax striatellus (Fallen) nymphs were unaffected when fed purified Cry1Na3 (250 mu g/ml) in their diet. Spodoptera exigua (Hubner) and Colaphellus bowringi (Baly) larvae survived even when the concentration of Cry1Na3 protein reached 500 mu g/ml. Cry1Na3 is a promising agent for the control of lepidopteran insect pests.

分类号： Q969.9

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