数字农科院2.0

Cloning and characterization of an S-RNase gene in Camellia sinensis

文献类型: 外文期刊

作者: Zhang, Cheng-Cai;Tan, Li-Qiang;Wang, Li-Yuan;Wei, Kang;Wu, Li-Yun;Zhang, Fen;Cheng, Hao;Zhang, Cheng-Cai;Tan, Li-Qiang;Wang, Li-Yuan;Wei, Kang;Wu, Li-Yun;Zhang, Fen;Cheng, Hao;Zhang, Cheng-Cai;Ni, De-Jiang;Tan, Li-Qiang

作者机构:

关键词: Camellia sinensis;Tea;Self-incompatibility;CsS-RNase;S-RNase;Genetic linkage map

期刊名称: SCIENTIA HORTICULTURAE

ISSN:

年卷期:

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收录情况: SCI

摘要: Self-incompatibility (SI) prevents inbreeding depression in angiosperms. Camellia sinensis is an important cash crop, but breeding improvements and genetic studies of the plant are hindered by SI. However, the SI mechanism in C. sinensis remains unclear. In this study, a putative S-RNase gene (KU852488) was cloned from C. sinensis. The full-length cDNA of CsS-RNase is 1121 bp, which encodes 238 amino acids. It shares the closest relationship with an S-RNase gene (ADA67883.1), which was cloned from a self incompatibility Citrus reticulata cultivar 'Wuzishatangju'. The expression level of CsS-RNase in the styles were 3-259 ('Fuding Dabaicha') and 5.6-119 ('Zhongcha108') times higher than the other tissues, for example petals, pollen grains, filaments and buds. And its expression rose in self-pollinated styles with 24h earlier than cross-pollinated styles. The genotypes of CsS-RNase in 10 cultivars and one breeding line of C. sinensis were analyzed. Totally, 11 polymorphic amino acid residues were identified. A single nucleotide polymorphism (SNP) marker of CsS-RNase was developed. Finally, the CsS-RNase was mapped onto a reference genetic linkage map of tea plant. (C) 2016 The Authors. Published by Elsevier B.V.

分类号: S6

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