数字农科院2.0

Cloning and characterization of thioredoxin peroxidases from Trichinella spiralis

文献类型： 外文期刊

作者： Zhang, N. Z.;Liu, J. Y.;Li, W. H.;Li, L.;Qu, Z. G.;Li, T. T.;Cui, J. M.;Yang, Y.;Jia, W. Z.;Fu, B. Q.;Jia, W. Z.;Fu, B. Q.

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关键词： Trichinella spiralis;Thioredoxin peroxidases;Antioxidant activity;Stage expression

期刊名称： VETERINARY PARASITOLOGY

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收录情况： SCI

摘要： The intracellular parasitic nematode, Trichinella spiralis, can initiate a high level of oxidative stress, especially during rapid growth and generative propagation phases. Thioredoxin peroxidases (TPXs) protect helminths against oxidative stress, but none has been identified in T. spiralis. Here, 3 members of the TPX family were cloned from T. spiralis muscle larvae (ML). The lengths of TsTPX ORFs were 747 bp, 588 bp and 594 bp, respectively, and the deduced proteins predicted to contain AhpC-TSA and 1-cys Prx_C domains. Interestingly, qRT-PCR data showed that TsTPX genes were expressed in all three developmental stages of T. spiralis. The TsTPX2 and TsTPX3 genes were up-regulated in day 3 adults (Ad3) compared with newborn larvae (NBL) and ML (P < 0.05); expression levels of the TsTPX1 gene in ML were higher compared with Ad3 and NBL amounts (P < 0.05). After prokaryotic expression, the reactivity of rTsTPX proteins was assessed by Western-blotting: only rTsTPX1 was specifically recognized by T. spiralis infection sera from pigs. Enzyme catalytic experiments showed that rTsTPX proteins could deoxidize H2O2 in the presence of OTT, with the catalytic ability increasing with protein concentration and time. (C) 2016 Elsevier B.V. All rights reserved.

分类号： S85

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