数字农科院2.0

Determination of soluble sugar profile in rice

文献类型： 外文期刊

作者： Hu, Xianqiao;Fang, Changyun;Lu, Lin;Hu, Zhanqiang;Shao, Yafang;Zhu, Zhiwei;Hu, Xianqiao;Fang, Changyun;Lu, Lin;Hu, Zhanqiang;Shao, Yafang;Zhu, Zhiwei

作者机构：

关键词： Soluble sugar;Rice;Ion chromatography;Pulsed amperometric detector

期刊名称： JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Soluble sugars in rice are the main components affecting sweetness taste of rice. In this paper, an accurate, precise and rapid method for simultaneous determination of multi soluble sugars in rice by using ion chromatography equipped with pulsed amperometric detector was presented. Pretreatment and parameters of ion chromatography and pulsed amperometric detector were optimized. Regression coefficients (R) of 0.9998, 1.0000, 0.9979, 0.9998 and 0.9998 were obtained for glucose, fructose, sucrose, raffinose and maltose, respectively. The recovery ranges of five sugars were 92.9-112.0% for milled rice matrix. Repeatability and reproducibility of the method were 0.8-9.7% and 1.9-7.6%, respectively. Method LODs of 3.1-34.6 mu g g(-1) were obtained for soluble sugars in milled rice matrix.

分类号： Q503

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