数字农科院2.0

Single-cell RNA sequencing reveals intrahepatic signature related to pathobiology of duck hepatitis A virus type 3 (DHAV-3) infection

文献类型： 外文期刊

作者： Yunsheng Zhang;Dingbang Ding;Shaofei Li;Qiong Pan;Jinlong Ru;Haonan Zhao;Guang Yao;Jie Wei;Shuaiqin Wang;Shuisheng Hou;Xia Wang

作者机构：

关键词： Coagulation;DHAV-3;duck;PLAC8;scRNA-seq

期刊名称： Poultry Science

ISSN： 0032-5791

年卷期： 2025 年 104 卷 2 期

页码：

收录情况： SCIE（2025版）

摘要： DHAV-3 is one of the main causative agents of duck viral hepatitis (DVH), an acute and highly lethal infectious disease in duck industry. However, the understanding of the pathogenesis of this virus in ducklings is limited. To dissect the molecular characteristics associated with pathobiology of ducklings to DHAV-3, we applied single-cell RNA-sequencing approach to profile the transcriptome of 1.4 million cells from 14 livers of DHAV-3 susceptible (S) and resistant (R) ducklings during viral infection and 4 uninfected healthy controls. We found that infected S ducks exhibited the activation of type I and II interferon pathways with elevated expression of interferon-stimulated genes (ISGs) compared to infected R ducks and healthy controls. DHAV-3 promoted proinflammatory phenotype and inhibited the cell apoptosis pathway of Kupffer cells of S ducks. Furthermore, we observed the elevated expression of host factor PLAC8 in S ducks and validated its ability to facilitate the infection of DHAV-3. We identified significant dysregulation of various genes in complement and coagulation cascades in hepatocytes2 exclusive to S ducks, together with over-secretion of ANGPTL4 from endothelial cells in S ducks which is confirmed to promote cellular migration, suggesting etiology of coagulopathic complications in ducks with severe DVH. Collectively, this study provides a rich resource for understanding the inflammatory immune signatures and cell communications underlying the pathogenesis of DHAV-3 infection, which may accelerate the development of better diagnostic methods and strategies for controlling this disease.

分类号：

  • 相关文献

[1]Polymorphisms of FUT9 and its relationship with susceptibility towards DHAV-3 in Pekin duck. Junting Cao,Jian Hu,Bo Zhang,Yunsheng Zhang,Zhiguo Wen,Yongbao Wu,Zhigang Hu,Zhengkui Zhou,Xiaolin Liu,Shuisheng Hou. 2025

[2]易感和抗性北京鸭感染DHAV-3后血浆生化指标的动态变化研究. 胡迪,梁锐萍,王霞,曹俊婷,邢光楠,王帅钦,侯水生,张云生. 2024

[3]DHAV-3感染对不同日龄雏鸭胆汁分泌通路的影响. 鲁美希,李慧慧,唐熠,梁素芸,侯水生. 2025

[4]Screening and analyzing genes associated with Amur tiger placental development. Li, Q.,Ma, J. Z.,Zhang, M. H.,Lu, T. F.,Hu, P. F.,Sun, B.,Wang, W. J.,Wang, K. F.,Zhang, W. X.,Guan, W. J.,Ma, Y. H.,Liu, D.,Chen, J.. 2014

[5]The performance of Fe2+/ClO− system in advanced removal of fulvic acid under mild conditions. Ting He,Liangwei Deng,Bo Lai,Shuguang Xu,Lan Wang,Yunhong Zhang,Dan Zheng,Changwei Hu. 2022

[6]Proteomics reveals the hemolymph components of partially fed Hyalomma asiaticum ticks. Yuan C.,Yang Q.,Wu J.,Peng Y.,Li Y.,Xiong S.,Zhou J.,Wang M.,Hu Z.,Zou Z.,Xia Q.. 2022

[7]NOD1 Is Associated With the Susceptibility of Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3. Suyun Liang,Ming Shan Wang,Bo Zhang,Yulong Feng,Jing Tang,Ming Xie,Wei Huang,Qi Zhang,Dabing Zhang,Shuisheng Hou. 2021

[8]Effect Of Duck Hepatitis A Virus Genotype 3 Infection On Glucose Metabolism Of Pekin Ducklings And Underlying Mechanism. Liang, SY, Tang, J, Wang, XY, Wen, ZG, Xie, M, Wu, YB, Hou, SS, Zhang, DB. 2020

[9]Plasma lipidome reveals susceptibility and resistance of Pekin ducks to DHAV-3. Xia Wang,Dingbang Ding,Ying Liu,Haonan Zhao,Jianfeng Sun,Yang Li,Junting Cao,Shuisheng Hou,Yunsheng Zhang. 2023

[10]Genomic Landscape and Regulation of RNA Editing in Pekin Ducks Susceptible to Duck Hepatitis A Virus Genotype 3 Infection. Zhao, Haonao,Wu, Zifang,Wang, Zezhong,Ru, Jinlong,Wang, Shuaiqin,Li, Yang,Hou, Shuisheng,Zhang, Yunsheng,Wang, Xia. 2024

[11]Effects of age on differential resistance to duck hepatitis A virus genotype 3 in Pekin ducks by 16 S and transcriptomics. Liang S.,Lu M.,Yu D.,Xing G.,Ji Z.,Guo Z.,Zhang Q.,Huang W.,Xie M.,Hou S.. 2024

[12]Effects of DHAV-3 infection on innate immunity, antioxidant capacity, and lipid metabolism in ducks with different DHAV-3 susceptibilities. Cao J.,Wen Z.,Zhang Y.,Zhang B.,Chen Y.,Xing G.,Wu Y.,Zhou Z.,Liu X.,Hou S.. 2024

[13]Whole-transcriptome sequencing revealed the role of noncoding RNAs in susceptibility and resistance of Pekin ducks to DHAV-3. Dingbang Ding,Haonan Zhao,Ying Liu,Shaofei Li,Jie Wei,Yuze Yang,Shuaiqin Wang,Guangnan Xing,Shuisheng Hou,Xia Wang,Yunsheng Zhang. 2024

[14]Host lipid metabolism influences the variation in resistance of Pekin ducks to duck hepatitis A virus genotype 3. Suyun Liang,Zhuo Chang,Meixi Lu,Zhanbao Guo,Dawei Luo,Guangnan Xing,Ming Xie,Wei Huang,Shuisheng Hou. 2025

[15]Early antioxidant capacity, intestinal barrier integrity and gut microbiota drive DHAV-3 resistance in ducks. Cao, Junting,Xu, Tong,Wu, Yongbao,Wang, Qimeng,Zhang, Bo,Yang, Yiwen,Guo, Yanhong,Zhang, Yunsheng,Zhou, Zhengkui,Hou, Shuisheng,Wen, Zhiguo. 2026

[16]Decoding the B-cell immune landscape in duck hepatitis A virus type 3 through single-cell genomics. Saisai Zhao,Yaru Fan,Dalin He,Mian Wu,Xinyuan Xu,Xinhong Man,Siming Zhu,Ruiqi Shi,Yafei Qin,Hong Yu Ma,Qiang Feng,Yi Tang,Youxiang Diao. 2025

[17]Global dissemination and evolutionary dynamics of duck hepatitis A virus type 3 driven by VP1 gene variation. Zhao, Saisai,Hao, Yizhang,Zhang, Qing,Li, Bing,Fan, Yaru,Liu, Guocheng,Li, Yiman,Zhang, Jiahao,Tang, Yi,Diao, Youxiang. 2025

[18]Stage-specific antiviral roles of IFIH1, IRF7, and MAVS in the interferon-mediated response to DHAV-3 infection. Huihui Li,Yi Tang,Shuisheng Hou,Suyun Liang. 2025

[19]Circular RNA circPTPN3 facilitates duck hepatitis A virus type 3 (DHAV-3) infection by reducing miR-130b-5p function in duck. Ying Liu,Shaofei Li,Xiang Mei,Shujie Tian,Junting Cao,Shuaiqin Wang,Shuisheng Hou,Xia Wang,Yunsheng Zhang. 2025

[20]SCSMRD: A database for single-cell skeletal muscle regeneration. Xi kang FENG,Chun di XIE,Yong yao LI,Zi shuai WANG,Li jing BAI. 2023

作者其他论文 更多>>