数字农科院2.0

Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis

文献类型: 外文期刊

作者: Wang, Du;Zhang, Zhaowei;Li, Peiwu;Zhang, Qi;Zhang, Wen;Zhang, Zhaowei;Li, Peiwu;Zhang, Qi;Zhang, Wen;Li, Peiwu;Zhang, Qi;Li, Peiwu;Wang, Du;Zhang, Wen

作者机构:

关键词: time-resolved fluorescent immunochromatographic strip;aflatoxin B1;soybean sauce;food safety

期刊名称: SENSORS

ISSN: 1424-8220

年卷期: 2016 年 16 卷 7 期

页码:

收录情况: SCI

摘要: Rapid and quantitative sensing of aflatoxin B1 with high sensitivity and specificity has drawn increased attention of studies investigating soybean sauce. A sensitive and rapid quantitative immunochromatographic sensing method was developed for the detection of aflatoxin B1 based on time-resolved fluorescence. It combines the advantages of time-resolved fluorescent sensing and immunochromatography. The dynamic range of a competitive and portable immunoassay was 0.3-10.0 mu g.kg(-1), with a limit of detection (LOD) of 0.1 mu g.kg(-1) and recoveries of 87.2%-114.3%, within 10 min. The results showed good correlation (R-2 > 0.99) between time-resolved fluorescent immunochromatographic strip test and high performance liquid chromatography (HPLC). Soybean sauce samples analyzed using time-resolved fluorescent immunochromatographic strip test revealed that 64.2% of samples contained aflatoxin B1 at levels ranging from 0.31 to 12.5 mu g.kg(-1). The strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique in food safety analysis.

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