数字农科院2.0

Analysis of Ribavirin and Its Metabolites in Fresh Eggs by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

文献类型： 外文期刊

作者： Qi Kai;Tang Xiao-Yan;Wang Min;Zheng Xin;Yang Meng-Rui;Zhou Jian;Qi Kai;Zheng Xin

作者机构：

关键词： Ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry;Ribavirin and its metabolites;Egg

期刊名称： CHINESE JOURNAL OF ANALYTICAL CHEMISTRY

ISSN： 0253-3820

年卷期： 2016 年 44 卷 6 期

页码：

收录情况： SCI

摘要： A rapid and convenient ultra high performance liquid chromatography. tandem mass spectrometry (UPLC-MS/MS) method for analysis of ribavirin and its two main kinds of metabolites, 1,2,4. triazole-3-carboxamide (TCONH2) and 1 beta D-ribofuranosyl-1,2,4-triazole-3-carboxylic acid (RTCOOH), in fresh eggs has been developed and validated. The sample was extracted with acetonitrile water (9. 1, V /V), added N. hexane to move liposoluble substances, after centrifugation, the upper solvent was cleaned. up by C-18 and GCB, then determined by UPLC-MS/MS coupled with Agilent ZORBAX SB. Aq column (100 mmx 3. 0 mm, 1.8 mu m). Over the concentration range of 2. 0-200. 0 mu g/L (0. 5-200. 0 mu g/L and 5. 0-200. 0 mu g/L) for ribavirin (TCONH2 and RTCOOH) in fresh egg matrix, the correlation coefficients (R-2) of the calibration curves were above 0. 99, and the limits of detection (LODs) were 0. 54 mu g/L (0. 09 and 1. 54 mu g/L). The limits of quantitation (LOQs) were 1. 79, 0. 31 and 5. 13 mu g/L, respectively. At three spiked concentration levels of 5. 0, 10. 0 and 50. 0 mu g/L, the recoveries of ribavirin and RTCOOH ranged from 96. 1% to 99. 6% and 42. 9% to 58. 3%. And at three spiked concentration levels of 0. 5, 2. 0 and 5. 0 mu g/L, the recoveries of TCONH2 ranged from 75. 9% to 106. 7%. The results of the determination in various real samples showed that the method is simple, accurate, rapid and suitable determination of ribavirin and its two main kinds of metabolites in fresh eggs.

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