数字农科院2.0

Transcriptome profiling of Cucumis melo fruit development and ripening

文献类型： 外文期刊

作者： Zhang, Hong;Yi, Hongping;Zhai, Wenqiang;Wang, Guangzhi;Wang, Huaisong;Fu, Qiushi

作者机构：

期刊名称： HORTICULTURE RESEARCH

ISSN： 2052-7276

年卷期： 2016 年 3 卷

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收录情况： SCI

摘要： Hami melon (Cucumis melo) is the most important melon crop grown in the north-western provinces of China. In order to elucidate the genetic and molecular basis of developmental changes related to size, flesh, sugar and sour content, we performed a transcriptome profiling of its fruit development. Over 155 000 000 clean reads were mapped to MELONOMICS genome, yielding a total of 23 299 expressed genes. Of these, 554 genes were specifically expressed in flowers, and 3260 genes in fruit flesh tissues. The 7892 differentially expressed genes (DEGs) were related to fruit development and mediated diverse metabolic processes and pathways; 83 DEGs and 13 DEGs were possibly associated with sucrose and citric acid accumulation, respectively. The quantitative real-time PCR results showed that six out of eight selected candidate genes displayed expression trends similar to our DEGs. This study profiled the gene expression related to different growing stages of flower and fruit at the whole transcriptome level to provide an insight into the regulatory mechanism underlying Hami melon fruit development.

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