数字农科院2.0

Bmo-miR-2758 Targets BmFMBP-1 (Lepidoptera: Bombycidae) and Suppresses Its Expression in BmN Cells

文献类型： 外文期刊

作者： Wang, Xin;Tang, Shunming;Song, Fei;Chen, Chen;Guo, Xijie;Shen, Xingjia;Wang, Xin;Tang, Shunming;Guo, Xijie;Shen, Xingjia

作者机构：

关键词： microRNA;Bombyx mori;Silk gland transcription factor;BmFMBP-1;Functional identification

期刊名称： JOURNAL OF INSECT SCIENCE

ISSN： 1536-2442

年卷期： 2016 年 16 卷

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are an abundant family of endogenous noncoding small RNA molecules. They play crucial roles on regulation of life processes both in plants and animals. Fibroin modulator binding protein-1 (FMBP-1) is a silk gland transcription factor of Bombyx mori, which is considered as a trans-activator of fibroin genes. And bioinformatics prediction showed that at the 3' untranslated region (3' UTR) of BmFMBP-1 there were binding sites for three bmo-miRNAs, bmo-miR-2b*, bmo-miR-305, and bmo-miR-2758, separately. In order to validate whether these bmo-miRNAs involved in the regulation of BmFMBP-1 expression, the expression levels of three bmo-miRNAs and BmFMBP-1 in the middle silk gland (MSG) and posterior silk gland (PSG) during the fourth-and fifth-larval stages of B. mori were measured by semi-quantitative reverse transcription polymerase chain reaction. The results revealed that the expression level of bmo-miR-2758 was the highest in the three, and it expressed higher in the PSG than in the MSG with a similar expression pattern as BmFMBP-1, implying that bmo-miR-2758 may involved in regulation of BmFMBP-1. To validate the regulation function of bmo-miR-2758 on BmFMBP-1, recombinant plasmids pcDNA3 [ie1-egfp-pri-bmo-miR-2758-SV40] and pGL3 [A3-luc-FMBP-1 3' UTR-SV40] were constructed and co-transfected in BmN cells. The dual-luciferase reporter assay system was used for assay of transient expression. The results showed that the expression of the luciferase reporter was significantly decreased when pGL3 [A3-luc-FMBP-1 3' UTR-SV40] co-transfected with pcDNA3 [ie1-egfp-pri-bmo-miR-2758-SV40] (P<0.01). Furthermore, when the artificial antisense RNA of bmo-miR-2758 (inhibitor) was added to the above co-transfection, the expression of the luciferase reporter was recovered significantly (P<0.01). These results suggest that bmo-miR-2758 represses the expression of BmFMBP-1 in vitro.

分类号：

  • 相关文献

[1]Computational identification and characteristics of novel microRNAs from the silkworm (Bombyx mori L.). Huang, Yong,Tang, Shun Ming,Wang, Li Gang,Shen, Xing Jia,Zou, Quan,Huang, Yong,Tang, Shun Ming,Wang, Li Gang,Shen, Xing Jia. 2010

[2]Characterization and profiling of MicroRNAs in posterior silk gland of the silkworm (Bombyx mori). Song, Fei,Wang, Xin,Chen, Chen,Fan, Yangyang,Tang, Shunming,Huang, Jinshan,Guo, Xijie,Shen, Xingjia,Tang, Shunming,Huang, Jinshan,Guo, Xijie,Shen, Xingjia.

[3]bmo-miR-0001 and bmo-miR-0015 down-regulate expression of Bombyx mori fibroin light chain gene in vitro. Chen, Chen,Fan, Yang-yang,Wang, Xin,Song, Fei,Jiang, Tao,Qian, Ping,Tang, Shun-ming,Shen, Xing-jia,Chen, Chen,Fan, Yang-yang,Wang, Xin,Song, Fei,Jiang, Tao,Qian, Ping,Tang, Shun-ming,Shen, Xing-jia. 2016

[4]GENOMIC ANALYSIS OF SILKWORM microRNA PROMOTERS AND CLUSTERS. Huang, Yong,Shen, Xing Jia,Huang, Jin Shan,Tang, Shun Ming,Huang, Yong,Shen, Xing Jia,Huang, Jin Shan,Tang, Shun Ming,Zou, Quan.

[5]Gene screening and differential expression analysis of microRNAs in the middle silk gland of wild-type and naked pupa mutant silkworms (Bombyx mori). Qian, Ping,Wang, Xin,Jiang, Tao,Song, Fei,Chen, Chen,Fan, Yangyang,Shen, Xingjia,Qian, Ping,Shen, Xingjia. 2016

[6]BMO-MIR-9A DOWN REGULATES THE EXPRESSION OF BM-ASE GENE IN VITRO. Song, Fei,Huang, Yong,Wang, Xin,Tang, Shunming,Shen, Xingjia,Shen, Xingjia,Huang, Yong.

[7]THE REGULATION OF SILKWORM Fibroin L CHAIN PRODUCTION BY miRNA-965 AND miRNA-1926 IN INSECT CELLS. Huang, Yong,Song, Fei,Wang, Xin,Shen, Xing Jia,Huang, Yong,Zou, Quan.

[8]The soybean GmDi19-5 interacts with GmLEA3.1 and increases sensitivity of transgenic plants to abiotic stresses. Feng, Zhi-Juan,Cui, Xiao-Yu,Cui, Xi-Yan,Chen, Ming,Yang, Guang-Xiao,Ma, You-Zhi,He, Guang-Yuan,Xu, Zhao-Shi. 2015

[9]Genome-wide analysis of the Hsf family in soybean and functional identification of GmHsf-34 involvement in drought and heat stresses. Li, Pan-Song,Chai, Shou-Cheng,Li, Pan-Song,Yu, Tai-Fei,He, Guan-Hua,Chen, Ming,Zhou, Yong-Bin,Xu, Zhao-Shi,Ma, You-Zhi. 2014

[10]Functional analysis of nodulin-like promoter in transgenic cotton plants. Ren, MZ,Chen, QJ,Li, L,Zhang, R,Guo, SD. 2005

[11]Functional analysis of a reproductive organ predominant expressing promoter in cotton plants. Ren, MZ,Chen, QJ,Li, L,Zhang, R,Guo, SD.

[12]Investigation of the ASR family in foxtail millet and the role of ASR1 in drought/oxidative stress tolerance. Feng, Zhi-Juan,Xu, Zhao-Shi,Li, Lian-Cheng,Chen, Ming,Ma, You-Zhi,Feng, Zhi-Juan,Sun, Jiutong,Yang, Guang-Xiao,He, Guang-Yuan.

[13]Functional identification of purine permeases reveals their roles in caffeine transport in tea plants (Camellia sinensis). Zhang Y.,Wei K.,Guo L.,Lei Y.,Cheng H.,Chen C.,Wang L.. 2022

[14]Genome-wide analysis of autophagy-associated genes in foxtail millet (Setaria italica L.) and characterization of the function of SiATG8a in conferring tolerance to nitrogen starvation in rice. 李微微,陈明,马有志. 2016

[15]Genome wide identification of GDSL gene family explores a novel GhirGDSL26 gene enhancing drought stress tolerance in cotton. Jiajun Liu,Jiangna Liu,Heng Wang,Aziz Khan,Yanchao Xu,Yuqing Hou,Yuhong Wang,Zhongli Zhou,Jie Zheng,Fang Liu,Xiaoyan Cai. 2023

[16]An Odorant Receptor from the Proboscis of the Cotton Bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) Narrowly Tuned to Indole. Guo, Mengbo,Ren, Xueting,Liu, Yang,Wang, Guirong. 2022

[17]Cloning and Functional Characterization of LlAS1 in Lilium lancifolium. Qu, Yuxiao,Yang, Panpan,Bi, Mengmeng,Xu, Leifeng,Ming, Jun. 2024

[18]益生菌干预对肥胖小鼠肝脏miR-33和miR-122表达的影响. 唐冬梅,郭艳杰,刘芳,王秋月,杨陈. 2019

[19]MicroRNA在治疗禽类病毒性感染中的应用. 高永伟,丛晓楠,孙学军,宋琳,王珊,洪炀. 2019

[20]小分子RNA表达载体构建的新方法—— MicroRNA前体PCR置换法. 陈锐,胡正,张辉. 2010

作者其他论文 更多>>