数字农科院2.0

A SNP-based molecular barcode for characterization of common wheat

文献类型: 外文期刊

作者: 高丽锋;;贾继增;;孔秀英

作者机构:

期刊名称: PLoS One

ISSN: 1932-6203

年卷期: 2016 年 11 卷 3 期

页码:

收录情况: JCR(2021版)

摘要: Wheat is grown as a staple crop worldwide. It is important to develop an effective genotyping tool for this cereal grain both to identify germplasm diversity and to protect the rights of breeders. Single-nucleotide polymorphism (SNP) genotyping provides a means for developing a practical, rapid, inexpensive and high-throughput assay. Here, we investigated SNPs as robust markers of genetic variation for typing wheat cultivars. We identified SNPs from an array of 9000 across a collection of 429 well-known wheat cultivars grown in China, of which 43 SNP markers with high minor allele frequency and variations discriminated the selected wheat varieties and their wild ancestors. This SNP-based barcode will allow for the rapid and precise identification of wheat germplasm resources and newly released varieties and will further assist in the wheat breeding program.

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