数字农科院2.0

De novo assembly and characterization of the transcriptome of the pancreatic fluke Eurytrema pancreaticum (trematoda: Dicrocoeliidae) using Illumina paired-end sequencing

文献类型： 外文期刊

作者： Liu, Guo-Hua;Xu, Min-Jun;Song, Hui-Qun;Zhu, Xing-Quan;Wang, Chun-Ren;Zhu, Xing-Quan

作者机构：

关键词： Eurytrema pancreaticum;Transcriptome;De novo;Next-generation sequencing

期刊名称： GENE

ISSN： 0378-1119

年卷期： 2016 年 576 卷 1 期

页码：

收录情况： SCI

摘要： Eurytrema pancreaticum is one of the most common trematodes living in the pancreatic and bile ducts of ruminants and also occasionally infects humans, causing eurytremiasis. In spite of its economic and medical importance, very little is known about the genomic resources of this parasite. Herein, we performed de novo sequencing, assembly and characterization of the transcriptome of adult E. pancreaticum. Approximately 36.4 million high-quality clean reads were obtained, and the length of the transcript contigs ranged from 66 to 19,968 nt with mean length of 479 nt and N50 length of 1094 nt, and then 23,573 unigenes were assembled. Of these unigenes, 15,353 (65.1%) were annotated by blast searches against the NCBI non -redundant protein database. Among these, 15,267 (64.8%), 2732 (11.6%) and 10,354 (43.9%) of the unigenes had significant similarity with proteins in the NR, NT and Swiss-Prot databases, respectively. 5510 (23.4%) and 4567 (19.4%) unigenes were assigned to GO and COG, respectively. 8886 (37.7%) unigenes were identified and mapped onto 254 pathways in the KEGG Pathway database. Furthermore, we found that 105 (1.18%) unigenes were related to pancreatic secretion and 61 (0.7%) to pancreatic cancer. The present study represents the first transcriptome of any members of the family Dicrocoeliidae, which has little genomic information available in the public databases. The novel transcriptome of E. pancreaticum should provide a useful resource for designing new strategies against pancreatic flukes and other trematodes of human and animal health significance. (C) 2015 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]De novo transcriptomic analysis of the female and male adults of the blood fluke Schistosoma turkestanicum. Chang, Qiao-Cheng,Wang, Chun-Ren,Liu, Guo-Hua,Xu, Min-Jun,Zhu, Xing-Quan,Xu, Min-Jun,Gao, Jun-Feng,Zhu, Xing-Quan. 2016

[2]De novo assembly and transcriptome analysis of two contrary tillering mutants to learn the mechanisms of tillers outgrowth in switchgrass (Panicum virgatum L.). Kaijie Xu,Fengli Sun,Guaiqiang Chai,Yongfeng Wang,Lili Shi,Shudong Liu,Yajun Xi. 2015

[3]siqRNA-seq is a spike-in-independent technique for quantitative mapping of mRNA landscape. Zhenzhen Wang,Kehan Tao,Jiaojiao Ji,Changbin Sun,Wei Xu. 2024

[4]Sequencing and characterization of the complete mitochondrial genome from the pancreatic fluke Eurytrema pancreaticum (Trematoda: Dicrocoeliidae). Chang, Qiao-Cheng,Zheng, Xu,Zhang, Yan,Duan, Hong,Yue, Dong-Mei,Fu, Xue,Su, Xin,Gao, Yuan,Wang, Chun-Ren,Liu, Guo-Hua,Gao, Jun-Feng.

[5]Identification and characterization of microRNAs in the pancreatic fluke Eurytrema pancreaticum. Xu, Min-Jun,Huang, Si-Yang,Fu, Jing-Hua,Zhou, Dong-Hui,Zhu, Xing-Quan,Wang, Chun-Ren,Chang, Qiao-Cheng,Zheng, Xu,Zhu, Xing-Quan,Fu, Jing-Hua,Zhu, Xing-Quan. 2013

[6]Comprehensive molecular characterization of a transgenic pig expressing hCD46 gene. Wang, Qin,Liu, Xiaofei,Fu, Wei,Lin, Xiangmei,Shi, Ningning,Pan, Dengke,Shi, Ningning,Shang, Ying,Shang, Ying,Xu, Wentao,Zhao, Yiqiang.

[7]De novo transcriptome sequencing and analysis of the juvenile and adult stages of Fasciola gigantica. Zhang, Xiao-Xuan,Ma, Jian-Gang,Zhao, Quan,Zhang, Xiao-Xuan,Cong, Wei,Ma, Jian-Gang,Zhu, Xing-Quan,Cong, Wei,Elsheikha, Hany M.,Liu, Guo-Hua,Huang, Wei-Yi,Zhu, Xing-Quan.

[8]Thirteen microsatellite loci for Laodelphax striatellus and cross amplification in related taxa. Liu, Yudi,Hou, Maolin,Zhang, Baoqin. 2014

[9]The complete chloroplast genome of Arabidopsis lyrata. Wu, Zhiqiang,Gu, Cuihua,Tembrock, Luke R.,Sun, Cheng. 2016

[10]Large-scale identification of microsatellites for a major invasive weed, Ageratina adenophora, using the Illumina sequencing technology. Liu, P. X.,Deng, P. C.,Lu, Y. Z.,Biradar, S. S.,Du, X. H.,Weining, S.,Nie, X. J.,Liu, P. X.,Deng, P. C.,Lu, Y. Z.,Biradar, S. S.,Du, X. H.,Weining, S.,Wu, A. P.,Wan, F. H..

[11]Effect of fumigation with chloropicrin on soil bacterial communities and genes encoding key enzymes involved in nitrogen cycling. Huang, Bin,Wang, Qiuxia,Li, Yuan,Fang, Wensheng,Yan, Dongdong,Guo, Meixia,Cao, Aocheng,Wang, Qiuxia,Li, Yuan,Yan, Dongdong,Guo, Meixia,Cao, Aocheng.

[12]Investigating the mechanisms of glyphosate resistance in goosegrass (Eleusine indica (L.) Gaertn.) by RNA sequencing technology. Huang, Hongjuan,Wei, Shouhui,Huang, Zhaofeng,Wang, Xu,Zhang, Chaoxian.

[13]Apple necrotic mosaic virus, a novel ilarvirus from mosaic-diseased apple trees in Japan and China. Yamagishi, Noriko,Yoshikawa, Nobuyuki,Yaegashi, Hajime,Ito, Tsutae,Xing, Fei,Li, Shifang,Xie, Jipeng,Zhou, Tao,Xie, Jipeng,Zhou, Tao.

[14]Comparative transcriptome analysis of genes involved in the response of resistant and susceptible peach cultivars to nematode infection. Cao, Ke,Li, Haiyan,Wang, Qi,Zhao, Pei,Zhu, Gengrui,Fang, Weichao,Chen, Changwen,Wang, Xinwei,Wang, Lirong.

[15]Effects of fumigation with metam-sodium on soil microbial biomass, respiration, nitrogen transformation, bacterial community diversity and genes encoding key enzymes involved in nitrogen cycling. Huang, Bin,Wang, Qiuxia,Li, Yuan,Fang, Wensheng,Han, Dawei,Yan, Dongdong,Guo, Meixia,Cao, Aocheng,Wang, Qiuxia,Li, Yuan,Yan, Dongdong,Guo, Meixia,Cao, Aocheng.

[16]The development of specific SNP markers for chromosome 14 in cotton using next-generation sequencing. WEI CHEN,JINBO YAO,LI CHU,YAN LI,XIANGMO GUO,YONGSHAN ZHANG. 2014

[17]Bacillus Amyloliquefaciens L-S60 Reforms The R.hizosphere Bacterial Community And I mproves Growth Conditions In Cucumber Plug Seedling. Qin, YX, Shang, QM, Zhang, Y, Li, PL, Chai, YR. 2017

[18]Virome Analysis Reveals Diverse and Divergent RNA Viruses in Wild Insect Pollinators in Beijing, China. Nannan Li,Yizhao Huang,Wei Li,Shufa Xu. 2022

[19]Virome Analysis for Identification of a Novel Porcine Sapelovirus Isolated in Western China. Chen, Jianing,Suo, Xuepeng,Cao, Liyan,Yuan, Cong,Shi, Lei,Duan, Yueyue,Zheng, Haixue,Wang, Qi. 2022

[20]Illumina sequencing of 18S/16S rRNA reveals microbial community composition, diversity, and potential pathogens in 17 turfgrass seeds. Li Ping Ban,Jin Dong Li,Min Yan,Yu Hao Gao,Jin Jin Zhang,Timothy W. Moural,Fang Zhu,Xue Min Wang. 2021

作者其他论文 更多>>