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Analysis of persistent changes to gamma-aminobutyric acid receptor gene expression in Plutella xylostella subjected to sublethal amounts of spinosad

文献类型：外文期刊

作者： Yin, X-H.;Long, Y-H.;Wu, X-M.;Li, R-Y.;Wang, M.;Tian, X-L.;Wu, Q-J.;Zhang, Y-J.;Jiao, X-G.

作者机构：

关键词： gamma-aminobutyric acid receptor gene;Multi-generation;Plutella xylostella;Spinosad;Sublethal effect

期刊名称： GENETICS AND MOLECULAR RESEARCH

ISSN： 1676-5680

年卷期： 2016 年 15 卷 3 期

页码：

收录情况： SCI

摘要： A multi-generational approach was used to investigate the persistent effects of a sub-lethal dose of spinosad in Plutella xylostella. The susceptibility of various sub-populations of P. xylostella to spinosad and the effects of the insecticide on the gene expression of gamma-aminobutyric acid receptor (GABAR) were determined. The results of a leaf dip bioassay showed that the sensitivity of P. xylostella to spinosad decreased across generations. The sub-strains had been previously selected based on a determined LC25 of spinosad. Considering that GABA-gated chloride channels are the primary targets of spinosad, the cDNA of P. xylostella was used to clone GABAR alpha by using reverse transcription-polymerase chain reaction (RT-PCR). The mature peptide cDNA was 1477-bp long and contained a 1449-bp open reading frame encoding a protein of 483 amino acids. The resulting amino acid sequence was used to generate a neighbor-joining dendrogram, and homology search was conducted using NCBI BLAST. The protein had high similarity with the known GABAR sequence from P. xylostella. Subsequent semi-quantitative RT-PCR and real-time PCR analyses indicated that the GABAR transcript levels in the spinosad-resistant strain (RR, 145.82-fold) and in Sub1 strain (selected with LC25 spinosad for one generation) were the highest, followed by those in the spinosad-susceptible strain, the Sub10 strain (selected for ten generations), and the Sub5 strain (selected for five generations). This multi-generational study found significant correlations between spinosad susceptibility and GABAR gene expression, providing insights into the long-term effects of sub-lethal insecticide exposure and its potential to lead to the development of insecticide-resistant insect populations.

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