数字农科院2.0

CRISPR/Cas9-mediated mutations of GS3 and GW5 positively regulate grain size and grain width in rice (Oryza sativa indica)

文献类型： 外文期刊

作者： Aqib Zeb;Amir Sohail;Shengjia Tang;Zhonghua Sheng;Peisong Hu

作者机构：

关键词： CRISPR/Cas9;Gene editing;Grain quality;Grain yield;GS3;GW5;Rice

期刊名称： Ecological Genetics and Genomics

ISSN： 2405-9854

年卷期： 2025 年 35 卷

页码：

收录情况： 无来源刊（2025版）

摘要： The enhancement of grain yield and quality through conventional breeding is a time-consuming and challenging process. Therefore, CRISPR/Cas9 technology is increasingly utilize to improve the grain yield and quality in rice. Grain size and weight are critical agronomic traits that influence both grain yield and quality, primarily governed by two key genes; grain size 3 (GS3) and grain width 5 (GW5). In this study, we constructed knockouts of GS3 and GW5 in Zhongjiazao17 (YK17) using a CRISPR/Cas9-mediated multiplex genome editing method. The gs3-1 knockout produced slender grains with a significant increase in grain length and grain size. Similarly, the gw5-2 knockout exhibited and enhanced thousand-grain weight. Both gs3-1 and gw5-2 knockouts showed a significant decrease in the number of panicle per plant and panicle length compared to YK17. Notably, both gs3-1 and gw5-2 knockouts also demonstrated a significant reduction in panicle length and panicle number. The gs3-1 knockout reduced amylose content (AC) and the highest gel consistency (GC), while gw5-2 exhibited higher AC and reduced GC compared to YK17. Additionally, the gs3-1 knockout also showed a significant decrease in grain chalkiness percentage. Taken together these results demonstrate that GS3 and GW5 are valuable targets for breeding programs aimed at improving grain yield and quality through CRISPR/Cas9 gene editing of functional alleles.

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