数字农科院2.0

Aflatoxin B1 and aflatoxin M1 induced cytotoxicity and DNA damage in differentiated and undifferentiated Caco-2 cells

文献类型： 外文期刊

作者： Zhang, J.;Zheng, N.;Li, S. L.;Wang, J. Q.;Zhang, J.;Li, F. D.;Zhang, J.;Zheng, N.;Li, S. L.;Wang, J. Q.;Liu, J.

作者机构：

关键词： Aflatoxin B1;Aflatoxin M1;Caco-2;Differentiated;Cytotoxicity;DNA damage

期刊名称： FOOD AND CHEMICAL TOXICOLOGY

ISSN： 0278-6915

年卷期： 2015 年 83 卷

页码：

收录情况： SCI

摘要： Aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) are natural mycotoxins that frequently present in food and feed and pose risks to human health. There are few data in the literature regarding the impairment of them in the intestine. Therefore, the present study investigated their cytotoxic effect on Caco-2 cells, especially the differentiated ones that resemble mature small intestinal enterocytes. Both undifferentiated (UC) and differentiated (DC) cells were treated with AFB1 and AFM1 at various concentrations for up to 72 h. Cell viability, lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) production and DNA damage were determined. Data showed that AFB1 and AFM1 significantly inhibited UC and DC cell growth, increased LDH and caused genetic damage in a time- and dose-dependent manner (p < 0.05). In comparison, AFB1 was found to be more toxic than AFM1 on both UC and DC. All these cytotoxic outcomes might be associated with intracellular ROS generation, leading to membrane damage and DNA strand break. Additionally, DC was found to be more sensitive to aflatoxins, which might be due to the alteration of enzymes during cell differentiation. The present study provided the first in vitro evidence of DNA damage of DC induced by AFB1 and AFM1. (C) 2015 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]Multi-Omics Reveal Additive Cytotoxicity Effects of Aflatoxin B1 and Aflatoxin M1 toward Intestinal NCM460 Cells. Ya Nan Gao,Xue Yang,Jia Qi Wang,Hui Min Liu,Nan Zheng. 2022

[2]Integrated Metabolomics and Lipidomics Analysis Reveals Lipid Metabolic Disorder in NCM460 Cells Caused by Aflatoxin B1 and Aflatoxin M1 Alone and in Combination. Xue Yang,Xue Li,Yanan Gao,Jiaqi Wang,Nan Zheng. 2023

[3]Omics analysis revealed the intestinal toxicity induced by aflatoxin B1 and aflatoxin M1. Ya Nan Gao,Zi Wei Wang,Chuan You Su,Jia Qi Wang,Nan Zheng. 2024

[4]黑酵母β-1,3/1,6-葡聚糖对感染肠炎沙门氏菌Caco-2细胞促炎症和抗炎症细胞因子基因mRNA表达水平的影响. 李晓敏,刘红宾,呙于明,王忠. 2013

[5]用体外消化/Caco-2细胞模型观察维生素C和柠檬酸对铁生物利用的影响. 雷激,张明秋,黄承钰,白琳,何中虎. 2008

[6]β-1,3/1,6-葡聚糖对肠炎沙门氏菌感染Caco-2细胞抗氧化功能的影响. 刘红宾,吴文郁,李晓敏,呙于明,王忠. 2014

[7]Impact of Lipid Phase on the Bioavailability of Vitamin E in Emulsion-Based Delivery Systems: Relative Importance of Bioaccessibility, Absorption, and Transformation. Yang, Ying,Yang, Ying,Xiao, Hang,McClements, David Julian.

[8]Isoflavone, gamma-aminobutyric acid contents and antioxidant activities are significantly increased during germination of three Chinese soybean cultivars. Wang, Fengzhong,Wang, Huifang,Wang, Donghui,Fang, Fang,Lai, Jixiang,Wu, Tao,Tsao, Rong.

[9]Dna Damage And Reactive Oxygen S.pecies Cause Cell Death I n The Rice Local Lesions 1 Mutant Under High Light And High Temperature. Zhang, Guangheng,Zhang, Qiang,Qiu, Zhennan,Zeng, Dali,Chen, Dongdong,Chen, Guang,Qian, Qian,Ren, Deyong,Zhu, Li,Hu, Jiang,He, Lei,Dong, Guojun,Shen, Lan,Guo, Longbiao,Gao, Zhenyu. 2019

[10]Sweet potato protein hydrolysates: antioxidant activity and protective effects on oxidative DNA damage. Mu, Miao Zhang Tai-Hua,Sun, Min-Jie. 2012

[11]FLUORIDE-INDUCED CELL CYCLE ARREST, APOPTOSIS, AND DNA DAMAGE IN TM3 MOUSE LEYDIG CELLS. Song, Chao,Yang, Zijun,Guo, Shuzhou,Shang, Zesong,Cao, Xiaodan.

[12]Assessment of genotoxic effects of flumorph by the comet assay in mice organs. Zhao, Q.,Ning, J.,Zhang, Y.. 2014

[13]Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain. Wei, Jie,Zhang, Xiao,Xu, Dexin,Zhang, Guokun,Gao, Jun,Fan, Jungang,Zhou, Zhiquan.

[14]Characterization of an ethylene-inducible, calcium-dependent nuclease that is differentially expressed in cucumber flower development. Gu, Hai-Tao,Wang, Dong-Hui,Li, Xia,Xu, Zhi-Hong,Bai, Shu-Nong,Gu, Hai-Tao,Wang, Dong-Hui,Xu, Zhi-Hong,Bai, Shu-Nong,He, Chao-Xing. 2011

[15]Oxidative Stress-Mediated Cytotoxicity of Cadmium in Chicken Splenic Lymphocytes. Li, Jin-Long,Tang, Zhao-Xin,Li, Jin-Long,Li, Shu,Xu, Shi-Wen,Li, Hui-Xin,Wang, Xiao-Long. 2010

[16]Oxidative stress of imidaclothiz on earthworm Eisenia fetida. Zhang, Lan,Feng, Lei,Mao, Liangang,Jiang, Hongyun.

[17]HDAC6 Triggers the ATM-Dependent DNA Damage Response To Promote PRV Replication. Xu, Weiyin,Yan, Ping,Zhou, Ziyan,Yao, Jingting,Pan, Haochun,Jiang, Luyao,Bo, Zongyi,Ni, Bo,Sun, Mingxia,Gao, Song,Huan, Changchao. 2023

[18]Integrated toxicity assessment of DEHP and DBP toward aquatic ecosystem based on multiple trophic model assays. Yuan, Lilai,Liu, Jia,Huang, Ying,Shen, Gongming,Pang, Sen,Wang, Chengju,Li, Yingren,Mu, Xiyan. 2022

[19]β-Catenin-Specific Inhibitor, iCRT14, Promotes BoHV-1 Infection-Induced DNA Damage in Human A549 Lung Adenocarcinoma Cells by Enhancing Viral Protein Expression. Xiuyan Ding,Weifeng Yuan,Hao Yang,Chang Liu,Shitao Li,Liqian Zhu. 2022

[20]Ecotoxicological effects of titanium dioxide nanoparticles and Galaxolide, separately and as binary mixtures, in radish (Raphanus sativus). Lisi Jiang,Qianru Zhang,Jianmei Wang,Wan Liu. 2021

作者其他论文 更多>>