数字农科院2.0

Genome-wide characterization and their roles in abiotic stress responses of HD-Zip transcription factors in pepper

文献类型： 外文期刊

作者： Xiaoqin Wang;Xiaoya Zhou;Kunhao Xie;Xiaojie Feng;LuLiu;Lihong Gao;Mintao Sun;Yansu Li;Chaoxing He

作者机构：

关键词： Abiotic stress;Capsicum annuum;Gene expression;HD-Zip gene family

期刊名称： BMC Plant Biology

ISSN： 1471-2229

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Background: Capsicum annuum is a globally cultivated crop of significant agricultural and economic importance. However, its productivity and fruit quality are frequently challenged by a range of abiotic stresses. The HD-Zip (Homeodomain-Leucine Zipper) gene family, unique to plants, is known to play pivotal regulatory roles in abiotic stress adaptation, yet its functional roles in pepper remain largely unexplored. Results: This study systematically analyzed the HD-Zip gene family in pepper through bioinformatics, expression profiling, and responses to abiotic stresses and phytohormones to elucidate their roles in stress tolerance. Results revealed 40 HD-Zip transcription factors unevenly distributed across 12 chromosomes, encoding proteins ranging from 211 to 842 amino acids. Subcellular localization predictions indicated nuclear localization for all members, with a subset also showing cytoplasmic localization. Collinearity analysis demonstrated that CaHD-Zip gene expansion was predominantly driven by segmental duplication, with high conservation across dicotyledons. Promoter regions of CaHD-Zip genes were enriched in cis-regulatory elements associated with light and hormonal responses, as well as stress adaptation. Tissue-specific and developmental stage-dependent expression patterns highlighted functional diversification within the family. Notably, some members were specifically induced by abiotic stresses (cold, heat, drought, and salt) and stress-related phytohormones (ABA, MeJA, ET, and SA), suggesting their involvement in stress signaling. Strikingly, CaHD-Zip18 and CaHD-Zip29 were significantly upregulated under all four stresses, implicating them as core regulators of multi-stress responses. Subsequent stress simulation assays and qRT-PCR validation confirmed the reliability of transcriptomic findings. Conclusion: This study delivers the first systematic exploration of HD-Zip transcription factors in Capsicum annuum under abiotic stress, providing foundational knowledge and candidate genes for improving stress resilience in pepper breeding programs.

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