数字农科院2.0

Interspecific Hybridization between Actinidia arguta and A. deliciosa and Embryo Culture In Vitro of Its F-1 Hybrids

文献类型： 外文期刊

作者： Qin, H. Y.;Ai, J.;Xu, P. L.;Liu, Y. X.;Zhao, Y.;Wang, Z. X.;Yang, Y. M.;Fan, S. T.;Li, X. Y.;Zhang, Q. T.

作者机构：

关键词： interspecific hybridization;embryo culture

期刊名称： VIII INTERNATIONAL SYMPOSIUM ON KIWIFRUIT

ISSN： 0567-7572

年卷期： 2015 年 1096 卷

页码：

收录情况： SCI

摘要： Interspecific hybidization was achieved beween Actinidia arguta (4x) females and A. deliciosa (6x) males, and hybrid embryos were cultured on a solidified Murashige and Skoog (MS) basal medium, supplemented with 16 different combinations of 3-indoleacetic acid (IAA), 6-benzylaminopurine (BAP) and gibberellins (GA). Best results were obtained when the F-1 embryos were cultured with supplementation with 1-naphthaleneacetic acid NAA (0.3 mg L-1), BAP (2 mg L-1) and GA (0.2 mg L-1). When the plantlets were cultured in medium with BAP (4 mg L-1) and NAA (0.01 mg L-1) significant improvement in proliferation was achieved within one month. Plantlets were rooted on 1/2 MS medium in combination with different concentrations of indole-3-butyric acid (IBA) or NAA, and high rooting and survival was achieved using 1/2 MS media with NAA (0.2 mg L-1). This study offered a new path for Actinidia breeding.

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