数字农科院2.0

Heterozygosities and genetic relationship of tea cultivars revealed by simple sequence repeat markers and implications for breeding and genetic mapping programs

文献类型: 外文期刊

作者: Tan, L. Q.;Qi, G. N.;Chen, S. X.;Zou, Y.;Zhang, C. C.;Wang, L. Y.;Wei, K.;Wu, L. Y.;Cheng, H.;Wang, L. Y.;Wei, K.;Cheng, H.

作者机构:

关键词: Tea cultivars;Heterozygosity;Genetic mapping;Simple sequence repeat marker

期刊名称: GENETICS AND MOLECULAR RESEARCH

ISSN: 1676-5680

年卷期: 2015 年 14 卷 1 期

页码:

收录情况: SCI

摘要: Genetic maps are essential tools for quantitative trait locus analysis and marker-assisted selection breeding. In order to select parents that are highly heterozygous for genetic mapping, the heterozygosity (H-S) of 24 tea cultivars (Camellia sinensis) was analyzed with 72 simple sequence repeat markers. In total, 359 alleles were obtained with an average of 4.99 per marker. The H-S varied greatly from 37.5 to 71.0% with an average of 51.3%. On average, tea cultivars from Fujian Province showed a higher level of heterozygosity (59.8%) than those from Zhejiang (48.5%) and Yunnan (44.5%), and the 12 national tea cultivars were generally more heterozygous than the 12 provincial cultivars. Unweighted pair-group analysis using the arithmetic average grouping divided the 24 cultivars into 2 groups that are consistent with the morphological classification. All dual combinations of the 24 cultivars were studied to calculate the percentage of mappable markers when using pseudo-testcross mapping strategy, and results showed that this value also varied greatly from 51.4 to 90.3%. The genetic relationships and H-S differences among different cultivars were discussed, and tea cultivars with high H-S were recommended as cross parents for genetic mapping programs.

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