Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis
文献类型: 外文期刊
作者: Zhang Fan;Wang Jian-Hua;Zhang Fan;Chen Xun-Ji;Zheng Jun;Chen Xun-Ji
作者机构:
关键词: maize;salt and drought stresses;ZmSnRK2.11;SnRK2
期刊名称: JOURNAL OF INTEGRATIVE AGRICULTURE
ISSN: 2095-3119
年卷期: 2015 年 14 卷 7 期
页码:
收录情况: SCI
摘要: Sucrose non-fermenting-1 related protein kinase 2 (SnRK2) is a unique family of protein kinases associated with abiotic stress signal transduction in plants. In this study, a maize SnRK2 gene ZmSnRK2.11 was cloned and characterized. The results showed that ZmSnRK2.11 is up-regulated by high-salinity and dehydration treatment, and it is expressed mainly in maize mature leaf. A transient expression assay using onion epidermal cells revealed that ZmSnRK2.11-GFP fusion proteins are localized to both the nucleus and cytoplasm. Overexpressing-ZmSnRK2.11 in Arabidopsis resulted in salt and drought sensitivity phenotypes that exhibited an increased rate of water loss, reduced relative water content, delayed stoma closure, accumulated less free proline content and increased malondialdehyde (MDA) content relative to the phenotypes observed in wild-type (WT) control. Furthermore, overexpression of ZmSnRK2.11 up-regulated the expression of the genes ABI1 and ABI2 and decreased the expression of DREB2A and P5CS1. Taken together, our results suggest that ZmSnRK2.11 is a possible negative regulator involved in the salt and drought stress signal transduction pathways in plants.
分类号:
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Association Study of Cooked Rice Textural Attributes and Starch Physicochemical Properties in indica Rice
作者:Deng Bowen;Zhang Yanni;Zhang Fan;Wang Wensheng;Xu Jianlong;Zhang Yu;Bao Jinsong
关键词:cooking and eating quality;genome-wide association study;rice;Waxy gene
-
Genetic dissection of heterosis of indica–japonica by introgression line, recombinant inbred line and their testcross populations
作者:Yang Wenqing;Zhang Fan;Zafar Sundus;Wang Junmin;Lu Huajin;Naveed Shahzad;Lou Jue;Xu Jianlong
关键词:
-
Multiomics analysis reveals the presence of a microbiome in the gut of fetal lambs
作者:Bi Yanliang;Tu Yan;Zhang Naifeng;Wang Shiqing;Zhang Fan;Suen Garret;Shao Dafu;Li Shengli;Diao Qiyu
关键词:intestinal microbiology
-
Multiomics analysis reveals the presence of a microbiome in the gut of fetal lambs
作者:Bi Yanliang;Tu Yan;Zhang Naifeng;Wang Shiqing;Zhang Fan;Suen Garret;Shao Dafu;Li Shengli;Diao Qiyu
关键词:intestinal microbiology
-
Multiomics analysis reveals the presence of a microbiome in the gut of fetal lambs
作者:Bi Yanliang;Tu Yan;Zhang Naifeng;Wang Shiqing;Zhang Fan;Suen Garret;Shao Dafu;Li Shengli;Diao Qiyu
关键词:intestinal microbiology
-
Identification and validation of root-specific promoters in rice
作者:Huang Li-yu;Zhang Fan;Qin Qiao;Wang Wen-sheng;Zhang Ting;Fu Bin-ying
关键词:rice;root-specific promoters;expression profile
-
Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice (Oryza sativa L.)
作者:Chai Lu;Zhang Jian;Zhang Fan;Zheng Tian-qing;Zhao Xiu-qing;Wang Wen-sheng;Xu Jian-long;Li Zhi-kang;Pan Xiao-biao;Jauhar, Ali
关键词:rice;whole plant growth duration salt tolerance;quantitative trait loci (QTLs);epistasis