数字农科院2.0

High Sensitive Time-Resolved Fluorescent Triplex Lateral Flow Test Strip for Quantitative Mycotoxin Detection in Foods and Feeds

文献类型： 外文期刊

作者： Chen, Kai;Tan, Ting;Zhang, Juan;Zhao, Wan;Ding, Li;Wang, Hai-Yu;Wang, Songxue;Zhang, Rui;Li, Aike;Xiao, Liwen;Chen, Ailiang

作者机构：

关键词： time-resolved fluorescence;lateral flow test strip;aflatoxin B1;zearalenone;deoxynivalenol

期刊名称： ACS FOOD SCIENCE & TECHNOLOGY

ISSN：

年卷期： 2025 年

页码：

收录情况： ESCI（2025版）

摘要： Mycotoxin cocontamination in food and feed poses significant health and economic risks, yet traditional methods often lack the sensitivity and throughput for simultaneous multitarget detection. This study presents a triplex lateral flow assay (TLFA) with three time-resolved fluorescent nanobead probes specific to aflatoxin B1 (AFB1), zearalenone (ZEN), and deoxynivalenol (DON), enabling their concurrent detection in complex food and feed matrices. The TLFA achieved detection limits of 0.86 ng/mL for AFB1, 14.02 ng/mL for ZEN, and 38.56 ng/mL for DON, benefiting from ultralow background fluorescence. The control (C) line, positioned near the sample pad and incorporating chicken IgY, provided a stable, independent quality-control signal. Validation with naturally contaminated samples from various matrices showed excellent agreement with high-performance liquid chromatography (HPLC), confirming the assay's accuracy and reliability. The entire workflow, including a simplified extraction step, is completed within 20 min, highlighting its potential for rapid, on-site mycotoxin monitoring in large-scale food and feed production.

分类号：

  • 相关文献

[1]Enzymatic degradation of multiple major mycotoxins by dye-decolorizing peroxidase from bacillus subtilis. Xing Qin,Xiaoyun Su,Tao Tu,Jie Zhang,Xiaolu Wang,Yaru Wang,Yuan Wang,Yingguo Bai,Bin Yao,Huiying Luo,Huoqing Huang. 2021

[2]Occurrence of mycotoxins in feed ingredients and complete feeds obtained from the Beijing region of China. Zhao, Lihong,Fan, Yu,Ma, Shanshan,Ji, Cheng,Ma, Qiugang,Zhang, Jianyun,Jia, Yaxiong,Sun, Lei.

[3]Mycotoxin contamination in organic and conventional cereal grain and products: A systematic literature review and meta-analysis. Juan Wang,Enas Khalid Sufar,Aksel Bernhoft,Chris Seal,Leonidas Rempelos,Gultekin Hasanaliyeva,Bingqiang Zhao,Per Ole Iversen,Marcin Baranski,Nikolaos Volakakis,Carlo Leifert. 2024

[4]Oxidative degradation and detoxification of multiple mycotoxins using a dye-decolorizing peroxidase from the white-rot fungus Bjerkandera adusta. Huimin Shao,Xiaoyun Su,Yaru Wang,Jie Zhang,Tao Tu,Xiaolu Wang,Huoqing Huang,Bin Yao,Huiying Luo,Xing Qin. 2024

[5]Effect of zearalenone on aflatoxin B1-induced intestinal and ovarian toxicity in pregnant and lactating rats. Kuntan Wu,Minjie Liu,Huanbin Wang,Shahid Ali Rajput,Omar Mahmoud Al Zoubi,Shuai Wang,Desheng Qi. 2023

[6]Simultaneous removal of aflatoxin B1 and zearalenone in vegetable oils by hierarchical fungal mycelia@graphene oxide@Fe3O4 adsorbent. Fei Ma,Qi Guo,Zhaowei Zhang,Xiaoxia Ding,Liangxiao Zhang,Peiwu Li,Li Yu. 2023

[7]Mixture toxic impacts and the related mechanism of aflatoxin B1 and deoxynivalenol on embryonic zebrafish (Danio rerio). Yanhua Wang,Qiang Wang,Chun Ji,Xiaoxuan Guo,Guiling Yang,Dou Wang,Hongbiao Weng,Yongzhong Qian,Chen Chen. 2021

[8]Yeast cell wall extract as a strategy to mitigate the effects of aflatoxin B1 and deoxynivalenol on liver and intestinal health, and gut microbiota of largemouth bass (Micropterus salmoides). Yu, Zhe,Zhang, Zhen,Teame, Tsegay,Guan, Leluo,Wang, Ruiguo,Zhu, Ronghua,Zhang, Qingshuang,Yang, Hongwei,Cui, Na,Huang, Yuan,Ran, Chao,Yang, Yalin,Su, Xiaoou,Zhou, Zhigang. 2025

[9]Evaluation of the combined impact of aflatoxin B1 and deoxynivalenol fed to Penaeus vannamei and mitigation properties provided by a yeast cell wall extract. Xiao Li,Jinzhu Yang,Weijian Huang,Gang Lin,Mingzhu Li,Kangsen Mai,Yanjiao Zhang. 2025

[10]Mycotoxin detection - Recent trends at global level. Selvaraj, Jonathan Nimal,Zhou Lu,Wang Yan,Zhao Yue-ju,Xing Fu-guo,Dai Xiao-feng,Liu Yang. 2015

[11]Recent mycotoxin survey data and advanced mycotoxin detection techniques reported from China: a review. Selvaraj, Jonathan Nimal,Wang, Yan,Zhou, Lu,Zhao, Yueju,Xing, Fuguo,Dai, Xiaofeng,Liu, Yang.

[12]Screening and monitoring zearalenone-producing Fusarium species by PCR and zearalenone by monoclonal antibodies in feed from China. Pei, Shi-Chun,Zhang, Hong-Fu,Ji, Cheng,Pei, Shi-Chun,Zhen, Yu-Ping,Gao, Jian-Wei,Lee, Won-Jong,Chen, Cong,Zhang, Xuan-Zhe.

[13]Cytotoxicity induced by ochratoxin A, zearalenone, and alpha-zearalenol: Effects of individual and combined treatment. Wang, H. W.,Wang, J. Q.,Li, S. L.,Zhang, Y. D.,Zheng, N.,Wang, H. W.,Li, F. D.,Wang, H. W.,Wang, J. Q.,Li, S. L.,Zhang, Y. D.,Zheng, N.,Zheng, B. Q.. 2014

[14]A novel detoxifying agent: Using rice husk carriers to immobilize zearalenone-degrading enzyme from Aspergillus niger FS10. He, Mengling,Li, Yun,Pi, Fuwei,Ji, Jian,He, Xingxing,Zhang, Yinzhi,Sun, Xiulan,Li, Yun.

[15]Biological detoxification of zearalenone by Aspergillus niger strain FS10. Sun, Xiulan,He, Xingxing,Li, Yun,Qian, He,Li, Yun,Xu, Dan,Xue, Kathy Siyu. 2014

[16]Rapid detection and quantification of zearalenone-producing Fusarium species by targeting the zearalenone synthase gene PKS4. Meng, Kun,Wang, Yaru,Yang, Peilong,Luo, Huiying,Bai, Yingguo,Shi, Pengjun,Yuan, Tiezheng,Ma, Rui,Yao, Bin. 2010

[17]Evaluation of reduced toxicity of zearalenone as measured by the Hep G2 cell assay on degradation enzymes. He, Xingxing,Li, Yun,Gu, Wenshu,Sun, Yange,Sun, Xiulan,Li, Shuai,Li, Yun.

[18]Multi-component immunochromatographic assay for simultaneous detection of aflatoxin B-1, ochratoxin A and zearalenone in agro-food. Li, Xin,Li, Peiwu,Zhang, Qi,Li, Ran,Zhang, Wen,Zhang, Zhaowei,Ding, Xiaoxia,Tang, Xiaoqian,Li, Xin,Zhang, Qi,Li, Ran,Zhang, Zhaowei,Li, Xin,Li, Peiwu,Li, Peiwu,Zhang, Zhaowei,Ding, Xiaoxia,Li, Peiwu,Zhang, Qi,Li, Ran,Zhang, Wen,Tang, Xiaoqian. 2013

[19]Selection Of A Dna Aptamer A.gainst Zearalenone And Docking A nalysis For Highly Sensitive Rapid Visual Detection With Label-Free Aptasensor. Zhang, YY, Lu, TF, Wang, Y, Diao, CX, Zhou, Y, Zhou, LL, Chen, HY. 2018

[20]Metabolic Profile Of Zearalenone In L.iver Microsomes From Different S pecies And Its In Vivo Metabolism In Rats And Chickens Using Ultra High-Pressure Liquid Chromatography-Quadrupole/Time-Of-Flight Mass Spectrometry. Yang, SP, Zhang, HY, Sun, FF, De Ruyck, K, Zhang, JZ, Jin, Y, Li, YS, Wang, ZH, Zhang, SX, De Saeger, S, Zhou, JH, Li, Y, De Boevre, M. 2017

作者其他论文 更多>>