数字农科院2.0

Multi-omics analyses reveal that DMDS-induced embryonic developmental arrest leads to eggshell damage and secondary suppression of chitin biosynthesis in Meloidogyne incognita

文献类型： 外文期刊

作者： 刘静怡;;王鑫;;孙小越;;李园;;方文生;;曹坳程;;王秋霞;;颜冬冬

关键词： Dimethyl disulfide (DMDS);;Meloidogyne incognita;;Ovicidal mechanism;;Chitin biosynthesis;;Glycosylation

期刊名称： Pesticide Biochemistry and Physiology

ISSN： 0048-3575

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Root-knot nematode (RKN) eggs have a multilayered structure that allows them to survive long-term in soil, serving as a major source of reinfestation. Dimethyl disulfide (DMDS), an efficient and environmentally friendly fumigant alternative to methyl bromide, shows significant inhibitory activity against RKN eggs, yet its ovicidal mechanism remains unclear. This study integrated microscopy, multi-omics, and biochemical assays to evaluate DMDS potency under contact and fumigation treatments. RT-qPCR and biochemical assays were used for validation. Results showed that the 24-h EC50 values of DMDS against eggs were 39.88 mg·L⁻¹ for contact treatment and 7.01 mg·L⁻¹ for Petri dish fumigation, while the 4-d EC50 for soil fumigation was 3.91 mg·kg⁻¹. Morphological analyses showed that DMDS treatment caused surface collapse and loss of structural integrity of the eggshell. Transcriptomic and proteomic analysis indicated significant downregulation of chitin synthase and chitin-binding protein genes, consistent with decreased chitinase activity. Furthermore, detoxification pathways, including glutathione metabolism and cytochrome P450- mediated detoxification, were significantly activated. The activities of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione S-transferase (GST), were markedly elevated. In summary, DMDS exerts a multifaceted toxicological effect on nematode eggs: it first penetrates into the egg and induces severe oxidative stress and energy-metabolic imbalance in the embryo, thereby jointly blocking normal embryonic development; this developmental arrest subsequently triggers secondary downstream effects, including disruption of eggshell structure and suppression of chitin biosynthetic processes. These findings provide a theoretical basis for the application of DMDS as a green and efficient fumigant for controlling plant-parasitic nematodes.

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