数字农科院2.0

Three Heat Shock Protein Genes from Bactrocera (Tetradacus) minax Enderlein: Gene Cloning, Characterization, and Association with Diapause

文献类型： 外文期刊

作者： Lu, Z. C.;Wang, L. H.;Zhang, G. F.;Wan, F. H.;Guo, J. Y.;Wan, F. H.;Guo, J. Y.;Wang, L. H.;Yu, H.;Wang, J. B.

作者机构：

关键词： Diapause;gene characterization;gene expression profile;heat shock protein

期刊名称： NEOTROPICAL ENTOMOLOGY

ISSN： 1519-566X

年卷期： 2014 年 43 卷 4 期

页码：

收录情况： SCI

摘要： Bactrocera (Tetradacus) minax Enderlein is a major pest to wild and cultivated species of citrus. Bactrocera minax produces one generation per year with a long pupal diapause period of over 6 months, which hinders efforts to obtain vast numbers of insects under standard room conditions. Determining the mechanisms of diapause is significantly important for obtaining large quantities of these insects. To characterize the heat shock protein (Hsp) genes of B. minax and to unravel their potential contribution to diapause, we performed 3' and 5' RACE to isolate the complementary DNA (cDNA) sequences, bioinformatics to examine the phylogenetic relationships, and real-time quantitative PCR to detect the expression patterns of three Hsp genes during various developmental stages. These results represent the first characterization of the three Hsp genes of B. minax; the open reading frames of Bmhsp23, Bmhsp70, and Bmhsp90 were 510, 1,911, and 1,089 bp, encoding 170, 636, and 363 amino acids, respectively. BmHsp70 and BmHsp90 displayed high identity to previously identified Hsp70 and Hsp90 genes, respectively. BmHsp23 displayed varying similarity, from 28 to 83%, to previously identified small Hsps. Bmhsp23 messenger RNA (mRNA) expression was found to be upregulated during diapause initiation, maintenance, and termination. Bmhsp70 mRNA expression peaked during diapause initiation. Bmhsp90 mRNA expression remained at a relatively low level during deep diapause. Our present results suggest that Bmhsp70 might play an important role in diapause initiation, while Bmhsp23 in diapause initiation and maintenance and Bmhsp90 in diapause regulation. These results improve our understanding of the mechanism of diapause in B. minax at the molecular level.

分类号：

  • 相关文献

[1]Isolation and characterization of a gene encoding a polyethylene glycol-induced cysteine protease in common wheat. Zang, Qing-Wei,Wang, Cai-Xiang,Li, Xu-Yan,Guo, Zhi-Ai,Jing, Rui-Lian,Chang, Xiao-Ping,Zhao, Jun.

[2]Aromatic Glucosinolate Biosynthesis Pathway in Barbarea vulgaris and its Response to Plutella xylostella Infestation. Liu, Tongjin,Zhang, Xiaohui,Yang, Haohui,Qiu, Yang,Wang, Haiping,Shen, Di,Song, Jiangping,Li, Xixiang,Agerbirk, Niels,Agerbirk, Niels. 2016

[3]Differential Gene Expression Profiles in Human THP-1 Monocytes Treated with Lactobacillus plantarum or Staphylococcus aureus Lipoteichoic Acid. Zeng, Ri-Zhong,Kim, Han Geun,Kim, Na Ra,Geun, Min,Chung, Dae Kyun,Zeng, Ri-Zhong,Kim, Han Geun,Kim, Na Ra,Geun, Min,Chung, Dae Kyun,Ko, Mi Yeon,Lee, Seung Yeon,Chung, Dae Kyun,Kim, Chul Min,Chung, Dae Kyun,Zeng, Ri-Zhong,Chung, Dae Kyun.

[4]Differential expression analysis of different albescent stages of 'Anji Baicha' (Camellia sinensis (L.) O. Kuntze) using cDNA microarray. Ma, Chun-Lei,Chen, Liang,Wang, Xin-Chao,Jin, Ji-Qiang,Ma, Jian-Qiang,Yao, Ming-Zhe,Wang, Zhi-Lan. 2012

[5]Expression profiling reveals functionally redundant multiple-copy genes related to zinc, iron and cadmium responses in Brassica rapa. Li, Jimeng,Liu, Bo,Cheng, Feng,Wang, Xiaowu,Wu, Jian,Li, Jimeng,Aarts, Mark G. M..

[6]Gene expression profiling during gland morphogenesis of a mutant and a glandless upland cotton. Cai, Yingfan,Xie, Yongfang,Mo, Jianchuan,Li, Shengwei,Jiang, Huaizhong,Pan, Zheng,Gao, Yunling,Chen, Min,He, Xiaohong,Yuan, Youlu,Shi, Yuzhen. 2010

[7]Identification and Characterization of Phytocyanin Family Genes in Cotton Genomes. Muhammad Bilal Tufail,Muhammad Yasir,Dongyun Zuo,Hailiang Cheng,Mushtaque Ali,Abdul Hafeez,Mahtab Soomro,Guoli Song. 2023

[8]Quail GHRL and LEAP2 gene cloning, polymorphism detection, phylogenetic analysis, tissue expression profiling and its association analysis with feed intake. Shu X.,Chen Z.,Zheng X.,Hua G.,Zhuang W.,Zhang J.,Chen J.. 2024

[9]Effects of maternal dietary manganese and incubation temperature on hatchability, antioxidant status, and expression of heat shock proteins in chick embryos. Zhu, Y. W.,Lu, L.,Li, W. X.,Zhang, L. Y.,Luo, X. G.,Zhu, Y. W.,Ji, C.,Lin, X.,Liu, H. C.,Odle, J..

[10]The effect of heat stress on gene expression and synthesis of heat-shock and milk proteins in bovine mammary epithelial cells. Hu, Han,Zhang, Yangdong,Zheng, Nan,Cheng, Jianbo,Wang, Jiaqi,Hu, Han,Zhang, Yangdong,Zheng, Nan,Cheng, Jianbo,Wang, Jiaqi,Hu, Han,Zhang, Yangdong,Zheng, Nan,Cheng, Jianbo,Wang, Jiaqi.

[11]Molecular cloning and functional analysis of the drought tolerance gene MsHSP70 from alfalfa (Medicago sativa L.). Li, Zhenyi,Long, Ruicai,Zhang, Tiejun,Wang, Zhen,Zhang, Fan,Yang, Qingchuan,Kang, Junmei,Sun, Yan.

[12]Alleviative effect of selenium on inflammatory damage caused by lead via inhibiting inflammatory factors and heat shock proteins in chicken testes. Wang, Yan,Wang, Kexin,Huang, He,Teng, Xiaohua,Gu, Xianhong.

[13]Over-expression of LlHsfA2b, a lily heat shock transcription factor lacking trans-activation activity in yeast, can enhance tolerance to heat and oxidative stress in transgenic Arabidopsis seedlings. Xin, Haibo,Zhong, Xionghui,Yi, Mingfang,Xin, Haibo,Zhang, Hua,Dong, Aixiang,Cong, Richen,Lian, Qinglong,Cao, Li.

[14]Characterization and binding analysis of a microneme adhesive repeat domain-containing protein from Toxoplasma gondii. Gong, Haiyan,Kobayashi, Kyousuke,Sugi, Tatsuki,Ishiwa, Akiko,Recuenco, Frances C.,Murakoshi, Fumi,Horimoto, Taisuke,Akashi, Hiroomi,Kato, Kentaro,Gong, Haiyan,Sugi, Tatsuki,Takemae, Hitoshi,Ishiwa, Akiko,Recuenco, Frances C.,Murakoshi, Fumi,Xuan, Xuenan,Kato, Kentaro. 2014

[15]cDNA-AFLP analysis reveals heat shock proteins play important roles in mediating cold, heat, and drought tolerance in Ammopiptanthus mongolicus. Guo, Huiming,Li, Zhaochun,Zhou, Meiliang,Cheng, Hongmei.

[16]Molecular cloning and characterization of the MsHSP17.7 gene from Medicago sativa L.. Li, Zhen-yi,Long, Rui-cai,Zhang, Tie-jun,Yang, Qing-chuan,Kang, Jun-mei.

[17]Molecular Cloning And Functional Analysis O.f The Drought Tolerance G ene Mshsp70 From Alfalfa (Medicago Sativa L.). Li, ZY, Long, RC, Zhang, TJ, Wang, Z, Zhang, F, Yang, QC, Kang, JM, Sun, Y. 2017

[18]Proteomic investigation of Peristenus spretus ovary and characterization of an ovary-enriched heat shock protein. . 2020

[19]Heat shock protein 70 and Cathepsin B genes are involved in the thermal tolerance of Aphis gossypii. Liu, Jinping,Liu, Yang,Li, Qian,Lu, Yanhui. 2023

[20]Bioinformatics Analysis, Expression Profiling, and Functional Characterization of Heat Shock Proteins in Wolfi-poria cocos. Hu, Xin,Tang, Xue,Zhou, Yumei,Ahmad, Bilal,Zhang, Deli,Zeng, Yue,Wei, Jingyi,Deng, Liling,Chen, Shijiang,Pan, Yu. 2023

作者其他论文 更多>>