数字农科院2.0

Monoclonal antibody-quantum dots CdTe conjugate-based fluoroimmunoassay for the determination of aflatoxin B-1 in peanuts

文献类型： 外文期刊

作者： Zhang, Zhaowei;Li, Yuanyuan;Li, Peiwu;Zhang, Qi;Zhang, Wen;Hu, Xiaofeng;Ding, Xiaoxia;Zhang, Zhaowei;Li, Yuanyuan;Li, Peiwu;Hu, Xiaofeng;Ding, Xiaoxia;Zhang, Qi;Zhang, Zhaowei;Li, Peiwu;Li, Peiwu;Zhang, Wen

作者机构：

关键词： Quantum dots CdTe;Fluoroimmunoassay;Aflatoxin B-1;Peanuts

期刊名称： FOOD CHEMISTRY

ISSN： 0308-8146

年卷期： 2014 年 146 卷

页码：

收录情况： SCI

摘要： A fluoroimmunoassay towards aflatoxin B-1 (AFB(1)) was presented using quantum dots as the fluorescent label. The CdTe QDs were successfully linked to the monoclonal antibody against AFB(1). Based on the conjugated complexes, a novel direct competitive fluorescence-linked immunosorbent assay (cFLISA) was developed for AFB(1) detection. The 50% inhibition value (IC50) of the cFLISA was 0.149 ng/mL in peanuts matrix. The method performance included the limit of detection (LOD) of 0.016 ng/mL and considerable recoveries of 85-117% at three fortification levels (0.075, 0.15, and 0.3 ng/g) from spiked AFB(1) blank peanuts samples, along with coefficients of variation (CVs) below 10%. The cFLISA provided an alternative of rapid and sensitive detection for AFB(1) and, moreover provided great potential for multiplexed mycotoxins determination simultaneously. (C) 2013 Elsevier Ltd. All rights reserved.

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