数字农科院2.0

Adjuvant effect enhancement of porcine interleukin-2 packaged into solid lipid nanoparticles

文献类型： 外文期刊

作者： Chen, Guohua;Zeng, Shuang;Jia, Huaijie;He, Xiaobing;Fang, Yongxiang;Jing, Zhizhong;Cai, Xuepeng

作者机构：

关键词： Recombinant porcine interleukine-2;Solid liquid nanoparticles;Packaging;Adjuvant effect

期刊名称： RESEARCH IN VETERINARY SCIENCE

ISSN： 0034-5288

年卷期： 2014 年 96 卷 1 期

页码：

收录情况： SCI

摘要： In this paper, we investigated the enhancement of adjuvant effects of porcine IL-2 (pIL-2) by packaging it into a solid lipid nanoparticle (SLN) delivery system. SLN-pIL-2 was prepared using hydrogenated castor oil and Polylactide-co-glycolide by double emulsion solvent evaporation methods (w/o/w). In animal trials, BALB/c mice were immunized with inactivated foot and mouth disease virus (FMDV) antigen combined with the SLN-pIL-2 adjuvant on days 0 and 14. Antibody titer, splenocyte proliferation, and secretion of IFN-gamma and IL-4 cytokines were determined. Our results showed that SLN-pIL-2 could significantly enhance FMDV-specific antibody level compared with recombinant pIL-2 alone (p < 0.05). In addition, SLN-pIL-2 significantly increased the proliferative responses of antigen-specific spleen cells. Furthermore, SLN-pIL-2 induced the secretion of IFN-gamma at a level higher than that induced by recombinant pIL-2 alone. Our results indicate that packaging recombinant pIL-2 in SLNs can be an effective way of boosting the effectiveness of pIL-2 as an adjuvant to enhance immune responses of vaccines. (C) 2013 Elsevier Ltd. All rights reserved.

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