数字农科院2.0

Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development

文献类型： 外文期刊

作者： Yang Ya-lan;Li Yan;Liang Ru-yi;Zhou Rong;Ao Hong;Mu Yu-lian;Yang Shu-lin;Li Kui;Tang Zhong-lin;Li Yan

作者机构：

关键词： porcine;microRNA-127;expression profiles;skeletal muscle development;target prediction

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2014 年 13 卷 6 期

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs), evolutionarily conserved non-coding RNAs in length 21-24 bp, play a critical role in skeletal muscle development. In this study, to explore the function of mircoRNA-127 in porcine skeletal muscle development, eight tissue samples from adult pigs and longissimus muscle samples at 26 developmental stages were collected from Tongcheng and Landrace pigs. The spatial-temporal expression profiles of miRNA-127 were carried out using step-loop quantitative real-time PCR (stem-loop RT-PCR). To explore the molecular functions of miRNA-127, we predicted its target genes and performed functional annotation using bioinformatics methods. Results suggested that miRNA-127 was abundantly expressed in heart, ovary, uterus and spleen tissues and was weakly expressed in liver, lung, kidney and small intestine in both Tongcheng and Landrace pigs. And miRNA-127 showed significant expression differences in heart, ovary, spleen and uterus tissues between these two breeds. miRNA-127 basically kept at a relatively stable high level in middle and later embryonic stages and a low expression level in early embryonic stages and postnatal stages, but the expression levels of miRNA-127 were higher in Tongcheng pigs than in Landrace at most developmental stages. miRNA-127 potentially regulated 240 candidate genes. Results of Gene Ontology and KEGG pathway analysis indicated that these genes could be involved in many molecular functions and mechanisms, such as regulation of the force of heart contraction, regulation of transcription, regulation of T cell differentiation, MAPK signaling pathway and GnRH signaling pathway. Many significantly enriched GO terms and KEGG pathways were related to skeletal muscle development. This study will be helpful to understand the biological function for miRNA-127 and identify candidate gene associated with meat production traits in pigs.

分类号：

  • 相关文献

[1]Characterization of MUSTN1 gene and its relationship with skeletal muscle development at postnatal stages in Pekin ducks. Xu, T. S.,Gu, L. H.,Liu, X. L.,Xu, T. S.,Xu, T. S.,Hou, S. S.,Zhang, X. H.,Sun, Y.,Ye, B. G.. 2015

[2]猪传染性胸膜肺炎PCR诊断方法的建立及初步应用. 王牟平,刘思国,王春来,刘杰,尹训南. 2003

[3]胸膜肺炎放线杆菌APXⅢA基因的克隆及原核表达. 贺英,逯忠新,石琴,赵萍,储岳峰,高鹏程. 2007

[4]应用SYBRGreenI实时荧光定量PCR检测Ⅱ型猪圆环病毒. 许洪喜,符芳,鲁晶红,李曦. 2007

[5]表达猪繁殖与呼吸综合征病毒GP5蛋白的重组伪狂犬病毒构建及其生物学特性分析. 田志军,仇华吉,倪健强,周艳君,蔡雪辉,孔令达,王云峰,童光志. 2003

[6]猪传染性胸膜肺炎放线杆菌血清型7型25-4株外膜蛋白(OMP)基因的克隆、序列分析和表达. 王牟平,刘思国,王春来,刘景利,尹训南. 2003

[7]The microRNAs in the antennae of Apolygus lucorum (Hemiptera: Miridae): Expression properties and targets prediction. Khashaveh A.,An X.,Shan S.,Pang X.,Li Y.,Fu X.,Zhang Y.. 2022

[8]Identification of miRNAs Involved in Olfactory Regulation in Antennae of Beet Webworm, Loxostege sticticalis (Lepidoptera: Pyralidae). Yu Zhang,Yanyan Li,Haibin Han,Xiaoling Wang,Shujing Gao,Qing Zhao,Halima Bieerdebieke,Linbo Xu,Qicong Zang,Hui Wang,Penghua Bai,Kejian Lin. 2024

[9]SMAD7, an antagonist of TGF-beta signaling, is a candidate of prenatal skeletal muscle development and weaning weight in pigs. Hua, Chaoju,Wang, Zishuai,Peng, Xing,Hou, Xinhua,Yang, Yalan,Li, Kui,Tang, Zhonglin,Li, Kui,Tang, Zhonglin,Zhang, Jianbing.

[10]Comprehensive analysis of lncRNAs involved in skeletal muscle development in ZBED6-knockout Bama pigs. Dandan Wang,Yabin Pu,Yefang Li,Dengke Pan,Shengnan Wang,Wenjie Tian,Yuehui Ma,Lin Jiang. 2021

[11]Effects of Equol Supplementation on Growth Performance, Redox Status, Intestinal Health and Skeletal Muscle Development of Weanling Piglets with Intrauterine Growth Retardation. Yong Zhang,Jingchang Ren,Li Chen,Honglin Yan,Tiande Zou,Hongfu Zhang,Jingbo Liu. 2023

[12]Multiomics analysis reveals signatures of selection and loci associated with complex traits in pigs. Liu, Lei,Yi, Guoqiang,Yao, Yilong,Liu, Yuwen,Li, Jiang,Yang, Yalan,Liu, Mei,Fang, Lingzhao,Mo, Delin,Zhang, Longchao,Liu, Yonggang,Niu, Yongchao,Wang, Liyuan,Qu, Xiaolu,Pan, Zhangyuan,Wang, Lei,Chen, Muya,Fan, Xinhao,Chen, Yun,Zhang, Yongsheng,Li, Xingzheng,Wang, Zhen,Tang, Yijie,Huang, Hetian,Yuan, Pengxiang,Liao, Yuying,Li, Xinjian,Yin, Zongjun,Liu, Di,Zhang, Dongjie,Zhou, Quanyong,Wu, Wangjun,Jiang, Jicai,Gao, Yahui,Liu, George E.,Wang, Lixian,Chen, Yaosheng,Li, Kui,Groenen, Martien A. M.,Tang, Zhonglin. 2024

[13]Multiomics analysis reveals signatures of selection and loci associated with complex traits in pigs. Liu, Lei,Yi, Guoqiang,Yao, Yilong,Liu, Yuwen,Li, Jiang,Yang, Yalan,Liu, Mei,Fang, Lingzhao,Mo, Delin,Zhang, Longchao,Liu, Yonggang,Niu, Yongchao,Wang, Liyuan,Qu, Xiaolu,Pan, Zhangyuan,Wang, Lei,Chen, Muya,Fan, Xinhao,Chen, Yun,Zhang, Yongsheng,Li, Xingzheng,Wang, Zhen,Tang, Yijie,Huang, Hetian,Yuan, Pengxiang,Liao, Yuying,Li, Xinjian,Yin, Zongjun,Liu, Di,Zhang, Dongjie,Zhou, Quanyong,Wu, Wangjun,Jiang, Jicai,Gao, Yahui,Liu, George E.,Wang, Lixian,Chen, Yaosheng,Li, Kui,Groenen, Martien A. M.,Tang, Zhonglin. 2025

[14]The toggle switch model for gene expression change during duck skeletal muscle development. Dingbang Ding,Lionel Kinkpe,Yang Li,Yunsheng Zhang,Xia Wang. 2025

[15]Single-cell transcriptional profiling reveals developmental dynamics of longissimus dorsi muscle in adult and Juvenile bactrian camels. Baojun De,Yiyi Liu,Lu Li,Fanhua Meng,Yuwen Liu,Ting Jia,Yuting Chen,Chunxia Liu,Shenyuan Wang,Tao Li,Hongmei Xiao,Fang Wan,Yingchun Liu,Wenlong Wang,Huanmin Zhou,Wenguang Zhang,Xin Wen,Jie Gong,A. Naer,Hongmei Bao,Qian Song,Ha Si Chaolu,Hai Long,Yanru Zhang,Junwei Cao. 2025

[16]Expression profiles of miRNAs in Gossypium raimondii. Jun MA,Teng-long GUO,Qing-lian WANG,Kun-bo WANG,Run-run SUN,Bao-hong ZHANG. 2015

[17]Identification and expression profile analysis of odorant-binding protein genes in Apolygus lucorum (Hemiptera: Miridae). Gu, Shao-Hua,Liu, Jing-Tao,Zhu, Xiao-Qiang,Guo, Yu-Yuan,Zhang, Yong-Jun,Liu, Jing-Tao,Zhou, Jing-Jiang.

[18]The Mouthparts Enriched Odorant Binding Protein 11 of the Alfalfa Plant Bug Adelphocoris lineolatus Displays a Preferential Binding Behavior to Host Plant Secondary Metabolites. Xiao, Qiang,Sun, Liang,Wei, Yu,Ma, Xiao-Yu,Xiao, Yong,Yang, Xian-Ming,Guo, Yu-Yuan,Zhang, Yong-Jun,Zhang, Dan-Dan,Zhang, Ya-Nan. 2016

[19]Genome-Wide Identification of the TIFY Gene Family in Brassiceae and Its Potential Association with Heavy Metal Stress in Rapeseed. Fujun Sun,Zhiyou Chen,Qianwei Zhang,Yuanyuan Wan,Ran Hu,Shulin Shen,Si Chen,Nengwen Yin,Yunshan Tang,Ying Liang,Kun Lu,Cunmin Qu,Wei Hua,Jiana Li. 2022

[20]Genome-wide analysis of R2R3-MYB transcription factors in Boehmeria nivea (L.) gaudich revealed potential cadmium tolerance and anthocyanin biosynthesis genes. Xinkang Feng,Aminu Shehu Abubakar,Kunmei Chen,Chunming Yu,Aiguo Zhu,Jikang Chen,Gang Gao,Xiaofei Wang,Pan Mou,Ping Chen. 2023

作者其他论文 更多>>