数字农科院2.0

Engineering of cry genes Cry11 and Cry1h in cotton (Gossypium hirsutum L.) for protection against insect pest attack

文献类型： 外文期刊

作者： Razzaq, Abdul;Ali, Arfan;Zahid, Sara;Malik, Arif;Pengtao, Li;Gong, Wankui;Youlu, Yuan;Ercisli, Sezai;Junaid, Muhammad Bilawal;Zafar, Muhammad Mubashar

作者机构：

关键词： Cotton transformation;Agrobacterium;Cry11;Cry1H;pink bollworms;army bollworms

期刊名称： ARCHIVES OF PHYTOPATHOLOGY AND PLANT PROTECTION

ISSN： 0323-5408

年卷期： 2023 年

页码：

收录情况： 不在遴选数据库范围（2023版）

摘要： Cotton is white gold and contributes significantly to the economy of the countries. The process of evolution of resistance in various cotton insects directly affects the life of people. Among the cotton insect pests, pink bollworm (Pectinophora gossypiella) and army bollworm (Spodoptera litura) have turned out to be destructive. The development of seed using gene stacking approach has tremendously reduced the applications of agrochemicals making the technology greener for society. A gene cassette containing Cry11 and Cry1H genes was constructed. The cassette was cloned into the pCAMBIA2300 plant expression vector using the CAMV3-35S promoter. The construct was transformed into cotton line FBS-222 using the shoot apex-cut Agrobacterium-mediated transformation method. The expression of the Cry genes was 3-fold higher in transgenic than the non-transgenic cotton plants using qRT-PCR. Finally, the insect bioassay with transgenic cotton showed 90% mortality against pink bollworms and 80% against cotton army bollworms. The results demonstrate the development of seeds using the gene pyramiding approach is an effective strategy to control insect pest attacks.

分类号：

  • 相关文献

[1]河南发生月季根癌病. 古勤生,冯红英,彭斌,王平格. 2004

[2]Transcriptome Analysis Of Maize Immature E.mbryos Reveals The Roles O f Cysteine In Improving Agrobacterium Infection Efficiency (Vol 8, 1778, 2017). Wang, Guoying,Zhang, Zhiqiang,Liu, Yunjun,Liu, Yan,Zhang, Zhiqiang,Fu, Junjie,Wang, Jianhua,Liu, Yan. 2017

[3]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[4]Establishment of a highly efficient transformation system for pepper (Capsicum annuum L.). Li, D,Zhao, K,Xie, B,Zhang, B,Luo, K. 2003

[5]Transcriptome Analysis Of Maize Immature E.mbryos Reveals The Roles O f Cysteine In Improving Agrobacterium Infection Efficiency. Liu, Y, Zhang, ZQ, Fu, JJ, Wang, GY, Wang, JH, Liu, YJ. 2017

[6]A highly efficient genetic transformation system for broccoli and subcellular localization. Yongyu Zhao,Dongxu Yang,Yumei Liu,Fengqing Han,Zhansheng Li. 2023

[7]Progress in Soybean Genetic Transformation Over the Last Decade. Xu, Hu,Guo, Yong,Qiu, Lijuan,Ran, Yidong. 2022

[8]Red fluorescence protein (DsRed2) promotes the screening efficiency in peanut genetic transformation. Dongxin Huai,Jie Wu,Xiaomeng Xue,Meiling Hu,Chenyang Zhi,Manish K. Pandey,Nian Liu,Li Huang,Dongmei Bai,Liying Yan,Yuning Chen,Xin Wang,Yanping Kang,Zhihui Wang,Huifang Jiang,Yong Lei,Rajeev K. Varshney,Boshou Liao. 2023

[9]Regulation Behavior Of Rsus-1 Promoter U.sing Uida Gene In R ice (Oryza Sativa). Ali, S, Zia, MA, Sun, HY, Shoukat, S, Shah, SH, Zhang, XY, Ali, GM. 2019

[10]Establishment of transformation system in wheat close relatives under assistance of TaWOX5. CHANG Ya-nan,LIU Jun-xian,LIU Chang,LIU Hui-yun,TANG Hua-li,QIU Yu-liang,LIN Zhi-shan,WANG Ke,YAN Yue-ming,YE Xing-guo. 2023

[11]Complete genome sequence of Agrobacterium fabrum ARqua1. Lan, Bo,Zhang, Qian,Yin, Kangquan. 2023

作者其他论文 更多>>