数字农科院2.0

Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents

文献类型： 外文期刊

作者： Tan Xiao-li;Zheng Xiang-feng;Zhang Zhi-yan;Wang Zheng;Xia Heng-chuan;Gu Shou-lai;Lu Chang-ming

作者机构：

关键词： Brasscia napus;LACS;lipid;subcellular localization;yeast

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2014 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Long-chain acyl-Coenzyme A (CoA) synthetases (LACSs) catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol (TAG) biosynthesis and fatty acid beta-oxidation. Here, we report the characterization of a LAGS gene from Brassica napus. It is highly homologous to Arabidopsis LACS4 and thus designated as BnLACS4. The.cloned gene BnLACS4 could complement a LAGS-deficient yeast strain YB525. It is mainly expressed in flowers and developing seeds where lipid biosynthesis is at high rate in Brassica napus. When transiently expressed in tobacco leaves, BnLACS4 is localized in endoplasmic reticulum (ER), the common site for eukaryotic pathway of lipid biosynthesis. Expression of BnLACS4 in the yeast strain pep4 increased its lipid content. Taken together, our results suggest that BnLACS4 may be involved in lipid biosynthesis in B. napus.

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