数字农科院2.0

Construction of a bacterial artificial chromosome library for Gossypium herbaceum var. africanum

文献类型： 外文期刊

作者： Gao HaiYan;WANG XingFen;LIU Fang;PENG RenHai;ZHANG Yan;CHENG Hua;WANG KunBo

作者机构：

关键词： wild cotton;Gossypium herbaceum var. africanum;bacterial artificial chromosome library;SSR marker;Verticillium wilt resistance

期刊名称： CHINESE SCIENCE BULLETIN

ISSN： 1001-6538

年卷期： 2013 年 58 卷 26 期

页码：

收录情况： SCI

摘要： Gossypium herbaceum var. africanum is the only wild cotton species within the cultivated diploid G. herbaceum. As the A sub-genome donor of tetraploid cotton, it is characterized by its resistance to insects, diseases, and other adversities. We have constructed the first bacterial artificial chromosome library (BAC) for G. herbaceum var. africanum. With high quality and broad coverage, this library includes 75000 clones, with an average insert size of about 115 kb and fewer than 4% empty clones. Our library is approximately five-fold the size of the A-genome (1667 Mb) and it provides 99.3% probability for isolating genes of interest or their sequences. Using nine SSR markers that are located on five different chromosomes and linked with resistance to Verticillium wilt, seven of nine could amplify the 40 superpools and got 1-14 hits. Because of its moderate wide coverage and relative large insert size, this library will be an important genomic resource for classifying and analyzing the evolution of cotton species, as well as for isolating disease-resistance genes and control elements.

分类号：

  • 相关文献

[1]Construction and characterization of a bacterial artificial chromosome library for the allotetraploid Gossypium tomentosum. F. Liu,Y.H. Wang,H.Y. Gao,C.Y. Wang,Z.L. Zhou,X.Y. Cai,X.X. Wang,Z.S. Zhang,K.B. Wang. 2015

[2]Construction and Characterization of a Bacterial Artificial Chromosome Library from the Huoyan Goose. Hu, Peng Fei,Li, Xiang Chen,Chen, Xian Wei,Guan, Wei Jun,Ma, Yue Hui. 2012

[3]Cloning and characterization of the Gossypium hirsutum major latex protein gene and functional analysis in Arabidopsis thaliana. Chen, Jie-Yin,Dai, Xiao-Feng.

[4]Genome-wide identification and analysis of a cotton secretome reveals its role in resistance against Verticillium dahliae. Ran Li,Xi Yue Ma,Ye Jing Zhang,Yong Jun Zhang,He Zhu,Sheng Nan Shao,Dan Dan Zhang,Steven J. Klosterman,Xiao Feng Dai,Krishna V. Subbarao,Jie Yin Chen. 2023

[5]Heterologous Expression Of The Cotton N.bs-Lrr Gene Gbana1 Enhances V erticillium Wilt Resistance In Arabidopsis. Li, NY, Zhou, L, Zhang, DD, Klosterman, SJ, Li, TG, Gui, YJ, Kong, ZQ, Ma, XF, Short, DPG, Zhang, WQ, Li, JJ, Subbarao, KV, Chen, JY, Dai, XF. 2018

[6]Genome-wide analysis of Hsp40 and Hsp70 gene family in four cotton species provides insights into their involvement in response to Verticillium dahliae and abiotic stress. Xin Zhou,Ling Su,Rui Tang,Yuxuan Dong,Fei Wang,Rong Li,Quanliang Xie,Xianliang Zhang,Guanghui Xiao,Hongbin Li. 2023

[7]Co-expression of multi-gene in cotton promotes the aggregation of multi-resistance and yield traits. Peng Wang,Hang Zhao,Xingxing Liu,Guilin Li,Xiaoshuang Zhang,Xueyan Zhang,Yadi Xing. 2023

[8]Uridine Kinase-like Protein (GhUKL4) Positively Regulates Resistance to Verticillium Wilt in Cotton. Baimei Cheng,Yanmeng Sun,Xiaohui Sang,Jianhua Lu,Pei Zhao,Wei Chen,Yunlei Zhao,Hongmei Wang. 2025

[9]Genome Wide SSR High Density Genetic Map Construction from an Interspecific Cross of Gossypium hirsutum X Gossypium tomentosum. Muhammad K.R.Khan,Haodong Chen,Zhongli Zhou,Muhammad K.Ilyas,Xingxing Wang,Xiaoyan Cai,Chunying Wang,Fang Liu,Kunbo Wang. 2016

[10]Unveiling the genetic landscape: Exploring the SSR-based genetic architecture and amino acid dissection of Gossypium barbadense and G. darwinii genomes. Allah Ditta,Zhongli Zhou,Muhammad Shehzad,Xiaoyan Cai,Yanchao Xu,Yuqing Hou,Fang Liu,Kunbo Wang,Muhammad K.R. Khan,Kotb A. Attia,Yaser M. Hafez,Arif A. Mohammed,Baohua Wang,Sajid Fiaz,Baohong Zhang. 2024

[11]Genetic insights into cold tolerance in cotton: GWAS identified GhPRL gene responsible for cold tolerance in cotton at seedling stage. Aamir Ali Abro,Mubashir Abbas,Qiankun Liu,Zheng Jie,Yanchao Xu,Yuqing Hou,Zhongli Zhou,Rashid Iqbal,Fang Liu,Xiaoyan Cai. 2025

[12]Genetic, metabolomic and transcriptomic analyses of the cotton yellow anther trait. Qian Liang,Xiaokang Feng,Daowu Hu,Yan Long Jin,Xuefeng Wang,Xiao Hu Ma,Rui Liang,Qian Hao Zhu,Shoupu He,Huaguo Zhu,Feng Liu,Xinyu Zhang,Jie Sun,Fei Xue. 2025

[13]Comparative Population Genomics of Relictual Caribbean Island Gossypium hirsutum. Weixuan Ning,Guanjing Hu,Daojun Yuan,Mark A. Arick,Chuan Yu Hsu,Zenaida V. Magbanua,Olga Pechanova,Daniel G. Peterson,Yating Dong,Joshua A. Udall,Corrinne E. Grover,Jonathan F. Wendel. 2026

[14]Genetic Analysis and Preliminary Mapping of a Highly Male-Sterile Gene in Foxtail Millet (Setaria italica L. Beauv.) Using SSR Markers. Wang Jun,An Yuan-huai,Guo Ping-yi,Wang Jun,Wang Zhi-lan,Yang Hui-qing,Yuan Feng,Guo Er-hu,Tian Gang,Li Hui-xia,Wang Yu-wen,Diao Xian-min. 2013

[15]Stripe rust resistance in Chinese bread wheat cultivars and lines. Xia, X. C.,Li, Z. F.,Li, G. Q.,He, Z. H.,Li, Z. F.,Li, G. Q.,Xia, X. C.,He, Z. H.,Singh, R. P.. 2007

[16]Analysis of simple sequence repeats markers derived from Phytophthora sojae expressed sequencetags. Zhu, ZD,Huo, YL,Wang, XM,Huang, JB,Wu, XF. 2004

[17]Mapping a resistance gene in wheat cultivar Yangfu 9311 to yellow mosaic virus, using microsatellite markers. Liu, WH,Nie, H,Wang, SB,Li, X,He, ZT,Han, CG,Wang, JR,Chen, XL,Li, LH,Yu, JL. 2005

[18]Genetic diversity and relationship of clonal tea (Camellia sinensis) cultivars in China as revealed by SSR markers. Fang, W.,Cheng, H.,Duan, Y.,Jiang, X.,Li, X.,Cheng, H.. 2012

[19]Analysis on Genetic Similarity of Japonica Rice Variety from Different Origins of Geography in the World. Shu Ai-ping,Cao Gui-lan,Han Long-zhi,Shu Ai-ping,Hwan, Kim Jong,Seong, Lee Kyu,Zhang San-yuan,Nan Zhong-hao. 2009

[20]Association mapping of dynamic developmental plant height in common wheat. Zhang, Jianan,Hao, Chenyang,Ren, Qian,Chang, Xiaoping,Jing, Ruilian,Zhang, Jianan,Zhang, Jianan,Liu, Guiru.

作者其他论文 更多>>