数字农科院2.0

Genetic analysis and location of gene for resistance to stripe rust in wheat international differential host Strubes Dickkopf

文献类型： 外文期刊

作者： Xu Jiao-Jiao;Lin Rin-Ming;Xu Shi-Chang;Xu Jiao-Jiao;He Yue-Qiu

作者机构：

关键词： wheat stripe rust;resistance gene;simple sequence repeat;YrSD

期刊名称： JOURNAL OF GENETICS

ISSN： 0022-1333

年卷期： 2013 年 92 卷 2 期

页码：

收录情况： SCI

摘要： Strubes Dickkopf is the sixth differential in the world set for wheat stripe (yellow) rust. It is very important to clarify its genetic character of resistance to stripe rust and to develop the molecular markers linked to resistance genes. The NIL Taichung 29*6/Strubes Dickkopf, which was obtained by Strubes Dickkopf as the gene donor and Taichung 29 as the genetic background through backcross breeding, was crossed with the recurrent parent Taichung 29, inbred, and backcrossed to obtain the F-1, F-2 and BC1 population. The genetic analysis of the cross Taichung 29/(Taichung 29*6/Strubes Dickkopf) was assessed by inoculating the rust race CYR26 at seedling stage. Bulked segregant analysis (BSA) and F-2 segregation analysis were used for detecting polymorphic primers to locate the gene. The resistance of the NIL Taichung 29*6/Strubes Dickkopf to CYR26 was controlled by a single dominant gene, named YrSD. The primer pair Xbarc59 on 5B was linked to YrSD and the genetic distance between Xbarc59 and YrSD was 2.4 cM. The molecular marker Xbarc59 closely linked to the gene YrSD could be used in marker-assisted selection for resistance to stripe rust in wheat breeding programmes.

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