数字农科院2.0

Identification of monoclonal antibody targeting epitope on p72 trimeric spike of African swine fever virus

文献类型： 外文期刊

作者： Duan, Xulai;Liu, Yingnan;Chen, Zongyan;Xie, Zhenhua;Tian, Chuanwen;Li, Yao;Lv, Lu;Wang, Rongrong;Liu, Jingyi;Chen, Hongjun

作者机构：

关键词： African swine fever virus (ASFV);p72 protein;Monoclonal antibody;Epitope

期刊名称： VIRUS GENES

ISSN： 0920-8569

年卷期： 2023 年

页码：

收录情况： SCIE（2023版）

摘要： African swine fever virus (ASFV) is highly contagious and can cause lethal disease in pigs. ASFV p72 protein is a major capsid protein that presents as trimer in the virion. Epitopes on the surface of p72 trimer are considered as protective antigens. In this study, recombinant p72 protein and p72-baculovirus were constructed and obtained. Three monoclonal antibodies (mAbs) specific to ASFV p72 protein, designated as 1A3, 2B5 and 4A5, were generated. Among them, 4A5 showed strong reactivity with ASFV infected cells. Subsequently, the epitope recognized by 4A5 was mapped and identified using a series of overlapping peptides generated from p72 protein. IFA and western blot analyses showed that 4A5 recognized the linear epitope of p72 monomer located between amino acids 245-285 and recognized the conformational epitope located at the surface and top of the p72 trimer. These findings will enrich our knowledge regarding the epitope on p72 protein and provide valuable information for further characterization of the antigenicity and molecular functions of p72 protein.

分类号：

  • 相关文献

[1]Identification of a linear B-cell epitope within the Bluetongue virus serotype 8 NS2 protein using a phage-displayed random peptide library. Qin, Yong-Li,Sun, En-Cheng,Liu, Ni-Hong,Yang, Tao,Xu, Qing-Yuan,Zhao, Jing,Wang, Wen-Shi,Wei, Peng,Feng, Yu-Fei,Li, Jun-Ping,Wu, Dong-Lai,Qin, Yong-Li,Sun, En-Cheng,Zhao, Jing,Wang, Wen-Shi,Wei, Peng,Li, Jun-Ping. 2013

[2]Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein. Han, Zongxi,Zhao, Fei,Shao, Yuhao,Liu, Xiaoli,Kong, Xiangang,Song, Yang,Liu, Shengwang.

[3]Identification of a linear epitope on the capsid protein of classical swine fever virus. Zhang, Xin,Sun, Yuan,Li, Su,Li, Na,Yang, Shuo,He, Fan,Huang, Jun-Hua,Ling, Li-Jun,Qiu, Hua-Ji,Xu, Jia. 2011

[4]Production and characterization of mouse monoclonal antibodies against lysis protein E of phiX174. Yu, Shenye,Zhao, Hailing,Wang, Huanan,Wang, Xiumei,Xu, Lili,Si, Wei,Chen, Liping,Zhang, Wanjiang,Liu, Siguo,Shao, Guangxi. 2013

[5]Identification of two antigenic epitopes on SARS-CoV spike protein. Hua, RH,Zhou, YJ,Wang, YF,Hua, YZ,Tong, GZ. 2004

[6]Identification of a conserved linear epitope on the VP1 protein of serotype O foot-and-mouth disease virus by neutralising monoclonal antibody 8E8. Yang, Decheng,Zhang, Chunyuan,Zhao, Lei,Zhou, Guohui,Wang, Haiwei,Yu, Li. 2011

[7]Identification of one novel epitope targeting p54 protein of African swine fever virus using monoclonal antibody and development of a capable ELISA. Yunlei Cao,Dongmei Han,Yujiao Zhang,Kuan Zhang,Nannan Du,Wu Tong,Guoxin Li,Hao Zheng,Changlong Liu,Fei Gao,Guangzhi Tong. 2021

[8]Expression of ASFV p17 in CHO cells and identification of one novel epitope using a monoclonal antibody. Liwei Li,Sina Qiao,Shumao Wang,Jiachen Liu,Kuan Zhao,Yanjun Zhou,Guoxin Li,Yifeng Jiang,Changlong Liu,Guangzhi Tong,Wu Tong,Fei Gao. 2023

[9]Identification of Two Linear Epitopes on MGF_110-13L Protein of African Swine Fever Virus with Monoclonal Antibodies. Shu Jian Zhang,Bei Niu,Shi Meng Liu,Yuan Mao Zhu,Dong Ming Zhao,Zhi Gao Bu,Rong Hong Hua. 2024

[10]Epitope Mapping of Senecavirus A 3A Protein Using Monoclonal Antibodies. Meng, Liang,Tian, Xiao-Xiao,Xiang, Xu-Yan,Qi, Xin-Yu,Zhou, Han-Rong,Xiao, Pei-Yu,An, Tong-Qing,Meng, Fan-Dan,Wang, Hai-Wei. 2025

[11]Development of an effective one-step double-antigen sandwich ELISA based on p72 to detect antibodies against African swine fever virus. Lei Wang,Duan Li,Yanlin Liu,Leyi Zhang,Guoliang Peng,Zheng Xu,Hong Jia,Changxu Song. 2023

[12]Function investigation of p11.5 in ASFV infection. Yin, Dan,Shi, Bin,Geng, Renhao,Liu, Yingnan,Gong, Lang,Shao, Hongxia,Qian, Kun,Chen, Hongjun,Qin, Aijian. 2024

[13]PI3K–Akt pathway-independent PIK3AP1 identified as a replication inhibitor of the African swine fever virus based on iTRAQ proteomic analysis. Bo Yang,Yu Hao,Jinke Yang,Dajun Zhang,Xijuan Shi,Xing Yang,Dengshuai Zhao,Wenqian Yan,Lingling Chen,Guohui Chen,Xintian Bie,Xiangtao Liu,Haixue Zheng,Keshan Zhang. 2023

[14]Transcriptome Profiling Reveals Features of Immune Response and Metabolism of Acutely Infected, Dead and Asymptomatic Infection of African Swine Fever Virus in Pigs. Hualin Sun,Qingli Niu,Jifei Yang,Yaru Zhao,Zhancheng Tian,Jie Fan,Zhonghui Zhang,Yiwang Wang,Shuxian Geng,Yulong Zhang,Guiquan Guan,David T. Williams,Jianxun Luo,Hong Yin,Zhijie Liu. 2021

[15]FoxJ1 inhibits African swine fever virus replication and viral S273R protein decreases the expression of FoxJ1 to impair its antiviral effect. Caina Ma,Shasha Li,Fan Yang,Weijun Cao,Huisheng Liu,Tao Feng,Keshan Zhang,Zixiang Zhu,Xiangtao Liu,Yonghao Hu,Haixue Zheng. 2022

[16]Establishment and Application of a Quantitative PCR Method for E248R Gene of African Swine Fever Virus. Li, Liwei,Du, Nannan,Chen, Jinxia,Zhang, Kuan,Tong, Wu,Zheng, Haihong,Zhao, Ran,Tong, Guangzhi,Gao, Fei. 2022

[17]LAMP assay coupled with CRISPR/Cas12a system for portable detection of African swine fever virus. Yang, Bo,Shi, Zhengwang,Ma, Yuan,Wang, Lijuan,Cao, Liyan,Luo, Juncong,Wan, Ying,Song, Rui,Yan, Yiyong,Yuan, Kehu,Tian, Hong,Zheng, Haixue. 2021

[18]Identification of African swine fever virus MGF505-2R as a potent inhibitor of innate immunity in vitro. Huaguo Huang,Wen Dang,Zhengwang Shi,Mingyang Ding,Fan Xu,Tao Li,Tao Feng,Haixue Zheng,Shuqi Xiao. 2023

[19]OGG1 inhibition suppresses African swine fever virus replication. Fan, Jie,Lv, Xinqian,Yang, Saixia,Geng, Shuxian,Yang, Jifei,Zhao, Yaru,Zhang, Zhonghui,Liu, Zhijie,Guan, Guiquan,Luo, Jianxun,Zeng, Qiaoying,Yin, Hong,Niu, Qingli. 2023

[20]ASFV transcription reporter screening system identifies ailanthone as a broad antiviral compound. Zhang, Yuhang,Zhang, Zhenjiang,Zhang, Fan,Zhang, Jiwen,Jiao, Jun,Hou, Min,Qian, Nianchao,Zhao, Dongming,Zheng, Xiaofeng,Tan, Xu. 2023

作者其他论文 更多>>