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The Influencing Factor of In Vitro Fertilization and Embryonic Transfer in the Domestic Fowl (Gallus domesticus)

文献类型： 外文期刊

作者： Li, B. C.;Li, W.;Zhang, Y. N.;Zhang, Z. T.;Wang, X. Y.;Gao, B.;Chen, H.;Dou, T. C.;Wang, K. H.

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期刊名称： REPRODUCTION IN DOMESTIC ANIMALS

ISSN： 0936-6768

年卷期： 2013 年 48 卷 3 期

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收录情况： SCI

摘要： Contents This study was conducted to explore the influencing factors of ova in vitro fertilization (IVF) and transfer of the fertilized ova into the oviduct of recipient hens. The efficiency of fertilization was compared using three aspects: (i) the different time of ova collection and transfer, (ii) egg-laying period of recipient hen; and (iii) semen volume. The following results are observed: 72%, 40% and 0% of ova were found in ovarian sac in 30 approximate to 40min, 50 approximate to 60min and more than 90min post-oviposition, respectively; 20%, 18%, 14% and 5.8% of ova were fertilized with 0.1, 0.2, 0.5 and 1.0ml semen, respectively; and 33% and 100% of healthy chickens were hatched from fertile ova with 0.1 and 0.5ml of semen, respectively. All oocytes obtained from ovary and mid-oviduct were unfertilized. Embryos were transferred into recipient hens 30min +/- 10min post-oviposition, and 70% of shelled eggs were produced. There were no eggs produced in the other transfer times. This demonstrated that live chicken can be obtained by IVF of ova collected shortly after oviposition. It was important that the ovum was transferred into the oviduct infundibulum of recipient hens immediately or shortly after oviposition.

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