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Detection of Neurotrophin-4 (NT-4) in Ejaculated Bull Spermatozoa and its Effect on Spermatozoa Mitochondrial Activity

文献类型： 外文期刊

作者： Wang, C-Q;Zhang, J.;Li, C-J;Li, W-H;Lu, C.;Liu, Z.;Wu, H.;Hou, X-F;Chen, C.;Zhou, X.;Wang, C-Q;Sun, Y-F;Wang, D.

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期刊名称： REPRODUCTION IN DOMESTIC ANIMALS

ISSN： 0936-6768

年卷期： 2013 年 48 卷 1 期

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收录情况： SCI

摘要： Contents Recently, we demonstrated that two members of neurotrophins, nerve growth factor and brain-derived neurotrophic factor, and two types of receptor, tyrosine kinase A (TrkA) and tyrosine kinase (TrkB), exist in ejaculated bull spermatozoa, and play a crucial role in the normal function of spermatozoa. Neurotrophin-4 (NT-4) is another neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase, and no reports of detection of NT-4 in spermatozoa have been published. In the present study, the presence of NT-4 in mature bull spermatozoa was investigated using RT-PCR, immunofluorescence and Western blotting. The result shows that there was no RT-PCR evidence for NT-4 transcripts in bovine spermatozoa. However, the NT-4 protein was present in bovine spermatozoa, and the NT-4 immunoreactivity was localized to the equatorial segment and midpiece of bovine spermatozoa. In addition, effects of NT-4 on function of spermatozoa were studied. Significant increased mitochondria activity of mature bovine spermatozoa was observed in response to 300 or 500 ng/ml exogenous NT-4 (p < 0.05), in comparison with the control, while addition of inhibitors (40 ng/ml k252a) specific for tyrosine protein kinase significantly blocked the increase of mitochondria activity. However, NT-4 had no effects on the viability or acrosome reaction of spermatozoa (p > 0.05). Consequently, this study provided evidence that NT-4 protein was presented in the mature bull spermatozoa and can influence the mitochondrial activity of bovine spermatozoa through TrkB tyrosine kinasedependent pathways.

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