数字农科院2.0

454 GS FLX Pyrosequencing Reveals Rumen Bacterial Diversity of Chinese Water Buffalo

文献类型： 外文期刊

作者： Yang, Chengjian;Yang, Bingzhuang;Zou, Caixia;Liang, Xin;Wei, Shengju;Li, Shulu;Liang, Xianwei;Luo, Hua

作者机构：

关键词： Chinese water buffalo;bacterial diversity;rumen;454 GS FLX pyrosequencing

期刊名称： BUFFALO BULLETIN

ISSN： 0125-6726

年卷期： 2013 年 32 卷

页码：

收录情况： SCI

摘要： In this study, high-throughput 16S rRNA gene-based pyrosequencing was used to investigate the rumen bacterial communities of Chinese water buffalo (swamp buffalo). Twelve female Chinese water buffaloes (Xilin buffalo, Haizi buffalo, Dehong buffalo, Dechan buffalo, three of each type) were used and given feed twice a day with forage ad libitum and four kg concentrate. Samples were taken from each water buffalo before morning feeding by stomach tube attached to hand air pump. Characterization of bacteria was achieved using V1-V3 hypervariable regions of the 16S rRNA gene by a 454 GS FLX PLUS system on twelve samples. Results showed that 21, 21, 20 and 18 bacterial phyla were identified in the ruminal microbiota of Xilin buffalo, Haizi buffalo, Dehong buffalo and Dechan buffalo, respectively. For whatever the buffalo type, rumen bacterial community was dominated by Bacteroidetes, Firmicutes and Proteobacteria. There were 227 genera of bacterial populations commonly shared by all twelve samples, including genera of Anaerovibrio, Bacteroides, Butyrivibrio, Lachnospiraceae, Pseudobutyrivibrio etc., indicating that there is a core microbial community in the microbial populations of swamp water buffaloes fed high forage diet. Lachnospiraceae, Prevotellaceae, Ruminococcaceae, Flavobacteriaceae, Veillonellaceae families were highly present and were clearly affected by buffalo types. The highest abundance of Prevotellaceae and the lowest abundance of Flavobacteriaceae were found in Haizi buffalo. The highest abundance of Lachnospiraceae and the lowest abundance of Veillonellaceae were found in Dechan buffalo. The highest abundance of Ruminococcaceae was found in Dehong buffalo. In conclusion, results of this study provided insights into the bacterial community structure and diversity of water buffalo and the bacterial taxa of Chinese water buffalo (swamp buffalo) appear to be phylogenetically related.

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