Purification of Transglutaminase from High Salt Culture by Gel and Cation Exchange Chromatography
文献类型: 外文期刊
作者: Zhang Li-Li;Zhang Lan-Wei;Du Ming;Han Xue;Yi Hua-Xi;Zhang Ying-Chun;Ma Wei;Zhang Yan-He
作者机构:
关键词: Transglutaminase;Purification;Liquid chromatography/tandem mass spectrometry;Gel chromatography;Cation exchange chromatography
期刊名称: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN: 0253-3820
年卷期: 2012 年 40 卷 8 期
页码:
收录情况: SCI
摘要: To purify the transglutaminase from high-salt medium, gel filtration combined with ion exchange chromatography method was employed. Two-step chromatographic conditions (flow rate and the sample volume) were optimized. The final sample volume and a flow rate for Gel chromatography were 6 mL and 0. 25 mL/min, respectively. Conditions for ion-exchange chromatography were a sample volume of 50 mL and flow rate of 3 mL/min. Enzyme was purified 4. 22-fold, the specific activity and the recovery rate were 17. 33 U/mg protein and 77. 5%, respectrively. A comparison between liquid chromatography/tandem mass spectrometric identification and the protein database was carried out and the results showed that the purified enzyme had high homology with AAN01353.
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