数字农科院2.0

A new strategy employed for identification of sweet orange cultivars with RAPD markers

文献类型： 外文期刊

作者： Sun, X.;Mu, Q.;Wang, C.;Wang, X. C.;Fang, J. G.;Jiang, D.

作者机构：

关键词： Sweet orange;RAPD;Cultivar identification;Molecular markers

期刊名称： GENETICS AND MOLECULAR RESEARCH

ISSN： 1676-5680

年卷期： 2012 年 11 卷 3 期

页码：

收录情况： SCI

摘要： We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar identification. Thirteen effective primers were first screened from 54 RAPD arbitrary 11-mer primers based on their amplification products and amplified polymorphic bands; they were then used for PCR amplification of all 60 cultivars. All cultivars were manually and completely separated by the polymorphic bands appearing in DNA fingerprints from 13 primers; a CID of the 60 sweet orange cultivars was then constructed. This CID separated all the cultivars from each other, based on the polymorphic bands; the corresponding primers were marked in the correct positions on the sweet orange CID. The CID strategy facilitates the identification of fruit cultivars with DNA markers. This CID of sweet orange cultivars will be very useful for the protection of cultivar rights and for early identification of seedlings in the nursery industry.

分类号：

  • 相关文献

[1]Prevalence of variation among American bollworm (Helicoverpa armigera) collected from different host plant in Punjab-Pakistan. Ali Raza,Muhammad Atif,Ammad Ramzan,Abu Bakar Umar Farooq,Ahsan Iqbal,Rao Sohail Ahmad Khan. 2022

[2]ASSOCIATION OF QUANTITATIVE TRAIT LOCI FOR PLANT HEIGHT WITH MAJOR DWARFING GENES IN RICE. Huang, N,Courtois, B,Khush, GS,Lin, HX,Wang, GL,Wu, P,Zheng, KL.

[3]Changes in free polyamines and expression of polyamine metabolic genes under drought and high-temperature in Citrus sinensis. Fu, X. Z.,Huang, Y.,Xing, F.,Chun, C. P.,Ling, L. L.,Cao, L.,Peng, L. Z.,Fu, X. Z.,Chun, C. P.,Ling, L. L.,Cao, L.,Peng, L. Z.,Huang, Y.,Xing, F..

[4]Genomewide Analysis Of The Ciii Peroxidase Family In Sweet Orange (Citrus Sinensis) And Expression Profiles Induced By Xanthomonas Citri Subsp. Citri And Hormones. Qin, Xiujuan,Chen, Shanchun,He, Yongrui,Li, Qiang,Dou, Wanfu,Qi, Jingjing. 2020

[5]Application of Herbal Plants in Organic Poultry Nutrition and Production. Shahrajabian M.H., Cheng Q., Sun W.. 2022

[6]DEVELOPMENT AND CHARACTERIZATION OF 20 NOVEL POLYMORPHIC STS MARKERS IN VICIA FABA (FAVA BEAN). Wang, Haifei,Yang, Tao,Guan, Jianping,Ma, Yu,Sun, Xuelian,Zong, Xuxiao.

[7]Molecular Characterization of Chinese Grape Landraces (Vitis L.) Using Microsatellite DNA Markers. Li, Beibei,Jiang, Jianfu,Fan, Xiucai,Zhang, Ying,Sun, Haisheng,Liu, Chonghuai,Li, Beibei,Zhang, Guohai.

[8]Melon2K array: A versatile 2K liquid SNP chip for melon genetics and breeding. Yu, Qing,Li, Shuai,Su, Xiaofeng,Chen, Xinxiu,Dong, Yuanhua,Yao, Zhiwang,Jiang, Naiyu,Chai, Sen,Zhang, Zhonghua,Xu, Kuipeng. 2025

[9]TEA5K: a high-resolution and liquid-phase multiple-SNP array for molecular breeding in tea plant. Liu, Dingding,Zhang, Chenyu,Ye, Yuanyuan,Mei, Piao,Gong, Yang,Liu, Zhen,Sun, Chao,Zhao, Xuecheng,Ding, Shiqi,Chen, Jiedan,Chen, Liang,Ma, Chunlei. 2025

[10]Development and application of a multiple nucleotide polymorphism (MNP)-based molecular identification system for pear cultivars. Yang, Xiang,Fang, Zhiwei,Cao, Yufen,Tian, Luming,Peng, Hai,Zhang, Ying,Xu, Jiayu,Zhou, Junfei,Huo, Hongliang,Qi, Dan,Liu, Chao,Li, Tiantian,Dong, Xingguang. 2025

[11]GeneticdiversityanalysisofMagnaporthegriseafromsomeblastnurseriesofHunanprovinceusingrandomamplifiedpolymorphicDNA. NguekoRB,沈瑛,王洪凯,林福呈,徐同. 2004

[12]Random amplified polymorphism of DNA analysis in Aegilops tauschii. Kong, LR,Dong, YC,Jia, JZ. 1998

[13]Assessment of genetic diversity among selected groundnut germplasm. I: RAPD analysis. Dwivedi, SL,Gurtu, S,Chandra, S,Yuejin, W,Nigam, SN. 2001

[14]RAPD study on inter-species relationships in Roegneria (Poaceae : Triticeae). Zhou, YH,Yang, JL,Zheng, YL,Yan, J,Jia, JZ,Wei, YM. 1999

[15]RAPD analysis of jasmine rice-specific genomic structure. Wu, Y. J.,Chen, Y.,Wang, J.,Zhu, C. X.,Xu, B. L.. 2006

[16]Phenotypic and genotypic changes in rapeseed after 18 years of storage and regeneration. Wu, XM,Wu, NF,Qian, XZ,Li, RG,Huang, FH,Zhu, L. 1998

[17]Morphological, cytological and molecular characterization of a novel symmetric somatic hybrid between N-tabacum and N-glauca. Sun, Y.,Xue, Q.,Zhang, X.,Ding, C.,Zhang, L.,Gong, M.,Bu, G.,Wang, W.. 2007

[18]IDENTIFICATION OF AN RAPD MARKER TIGHTLY LINKED TO BLAST RESISTANCE IN ORYZA-SATIVA L. LU, J,QIAN, HR,ZHUANG, JY,LIN, HX,ZHENG, KL. 1995

[19]Strain-typing of Lentinula edodes in China with inter simple sequence repeat markers. Zhang, Ruiying,Huang, Chenyang,Zheng, Suyue,Zhang, Jinxia,Ng, Tzi Bun,Jiang, Ruibo,Zuo, Xuemei,Wang, Hexiang.

[20]Quantitative trait loci underlying the development of seed composition in soybean (Glycine max L. Merr.). Li, Wenbin,Sun, Desheng,Du, Yuping,Chen, Qingshan,Zhang, Zhongchen,Qiu, Lijuan,Sun, Genlou.

作者其他论文 更多>>