Allelic Variation at the TaZds-A1 Locus on Wheat Chromosome 2A and Development of a Functional Marker in Common Wheat
文献类型: 外文期刊
作者: Dong Chang-hai;Ma Zhi-ying;Dong Chang-hai;Xia Xian-chun;Zhang Li-ping;He Zhong-hu;He Zhong-hu;Zhang Li-ping
作者机构:
关键词: Triticum aestivum;functional marker;QTL;zeta-carotene desaturase;ZDS
期刊名称: JOURNAL OF INTEGRATIVE AGRICULTURE
ISSN: 2095-3119
年卷期: 2012 年 11 卷 7 期
页码:
收录情况: SCI
摘要: zeta (zeta)-carotene desaturase (ZDS) is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment (YP) content in wheat grain. Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2A, designated TaZds-A1, was cloned, with 14 exons and 13 introns, and it has an open reading frame (ORF) of 1 707 bp, encoding 568 amino acid residues. A co-dominant functional marker, YP2A-1, was designed based on the polymorphisms of two alleles at the locus, TaZds-A1a and TaZds-A1b, yielding 183- and 179-bp fragments in TaZds-A1 a and TaZds-A1b genotypes, respectively. A new QTL for YP content was detected on chromosome 2A, co-segregating with the functional marker YP2A-1 and TaZds-A1; it explained 11.3% of the phenotypic variance for YP content in a doubled haploid (DH) population from Zhongyou 9507/CA9632. Among 217 Chinese wheat cultivars and advanced lines, the average grain YP content of 126 cultivars with TaZds-A1b allele was 7.8% higher than that of 91 cultivars with TaZds-A1a allele.
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