数字农科院2.0

A Simple Method for Isolating Chloroplast DNA and Mitochondria DNA from the Same Rapeseed Green Leaf Tissue

文献类型： 外文期刊

作者： Hu Zhi-yong;Zhan Gao-miao;Wang Han-zhong;Hua Wei

作者机构：

关键词： chloroplast DNA;mitochondrial DNA;organelle isolation;rapeseed

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2012 年 11 卷 7 期

页码：

收录情况： SCI

摘要： In the study, we present a fast, simple and inexpensive protocol for isolating chloroplast and mitochondrial DNA from one rapeseed leaf tissue sample. The chloroplast and mitochondria were separated from the same green leaf tissue by differential centrifugations. The protocol is the first report that isolates plant chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) from the same sample homogenate. The organelle DNA yield is 2-10 micrograms per gram of tissue; the DNA was fully restrictable and was successfully used for sequencing.

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