数字农科院2.0

Strategies to Improve γ-Aminobutyric Acid Biosynthesis in Rice via Optimal Conditions

文献类型： 外文期刊

作者： Lixing Shen;Yulu Yang;Xiong Liu;Huibo Zhao;Yanfang Zhang;Lan Shen;Li Zhu;Jiang Hu;Deyong Ren;Qiang Zhang;Zhenyu Gao;Guojun Dong;Qing Li;Qian Qian;Dali Zeng;Guangheng Zhang

作者机构：

关键词： amino acid content;embryo;enrichment conditions;germinated;rice (Oryza sativa L.);γ-aminobutyric acid (GABA)

期刊名称： Plants

ISSN： 2223-7747

年卷期： 2025 年 14 卷 9 期

页码：

收录情况： SCIE（2025版）

摘要： The γ-aminobutyric acid (GABA), a ubiquitous non-protein amino acid in plants and animals, exhibits diverse biological activities and holds promise in human disease prevention and treatment. Prior studies have shown that germination could substantially elevate GABA levels in rice, but these investigations typically focused on limited germplasms, hindering the generalization of their findings. This study aims to identify optimal conditions for enriching GABA in a diverse set of 225 rice germplasms by examining the effects of various germination times, temperatures, and soaking solution pH levels, while elucidating the key factors influencing GABA enrichment in germinated brown rice. The optimal GABA enrichment in germinated brown rice was achieved under the following conditions: a germination temperature of 37 °C, a germination duration of 48 h, and a soaking solution pH of 5.5. Under these conditions, we found significant differences in GABA content among different germplasms. Subsequent correlation analyses demonstrated that GABA content showed significant positive correlations with embryo weight in brown rice, relative embryo weight, relative embryo weight in germinated brown rice, as well as glutamate (Glu) and proline (Pro) concentrations. Therefore, larger brown rice embryos, higher Glu and Pro content in germinated brown rice, and external Glu application contribute to increased GABA content. Our findings provide essential materials and theoretical insights for screening and developing GABA-rich functional rice germplasms, facilitating variety selection and breeding programs.

分类号：

  • 相关文献

[1]Genetic Dissection for Leaf Correlative Traits of Rice (Oryza sativa L.)Under Drought Stress. Guo Longbiao,Qian Qian,Zeng Dali,Dong Guojun,Teng Sheng,Zhu Lihuang. 2004

[2]TheDifferentialTranscriptionNetworkbetweenEmbryoandEndospermintheEarlyDevelopingMaizeSeed. XiaoduoLu,DijunChen,DefengShu,ZhaoZhang,WeixuanWang,ChristianKlukas,Ling-lingChen,YunliuFan,MingChen,ChunyiZhang. 2015

[3]Nutritional components and antioxidant activities of Firmiana platanifolia seeds in response to different germination times. Xi Min Zhi,Wen Zhang Qian,Yi Song,Chen Jun Mu,De Shui Yu,Xi Lin,Shun Gao. 2024

[4]QTL detection of amino acid content in grains of rice using advanced backcross introgression lines. Cheng, Li-Rui,Luo, Cheng-Gang,Xu, Jian-Long. 2013

[5]Association Analysis of the Amino Acid Contents in Rice. Zhao, Weiguo,Chung, Jong-Wook,Park, Yong-Jin,Zhao, Weiguo,Park, Eun-Jin,Chung, Ill-Min,Ahn, Joung-Kuk,Kim, Gwang-Ho,Zhao, Weiguo. 2009

[6]Pleiotropic effects of rice florigen gene RFT1 on the amino acid content of unmilled rice. 谢黎虹,,朱玉君,,唐绍清,,魏祥进,,圣忠华,,焦桂爱,,胡培松,,庄杰云. 2020

[7]Identification of Candidate Genes that Affect the Contents of 17 Amino Acids in the Rice Grain Using a Genome-Wide Haplotype Association Study. Xiaoqian Wang,Lihong Xie,Jiachuang Fang,Yunlong Pang,Jianlong Xu,Zhikang Li. 2023

[8]The investigation into the physicochemical properties of fermented soybean meal and its effects as a substitute for soybean meal on the growth performance and intestinal development of broiler chickens. Manqi Zhao,Xing Chen,Shuang Li,Guohua Liu,Zhimin Chen,Peilong Yang,Kun Meng,Aijuan Zheng. 2025

[9]A Comparison of in vitro Development of Buffalo (Bubalus bubalis) Embryos Produced either by Somatic Cell Nuclear Transfer or in vitro Fertilization using Oocytes Obtained by Different Methods. Yang, Chunyan,Pang, Chunying,Zheng, Haiying,Huang, Fenxiang,Shang, Jianghua,Wang, Jian,Yang, Bingzhuang,Liang, Xianwei. 2013

[10]Effects of Curcumin on Buffalo Embryonic Development In Vitro. Shang, Jiang-Hua,Yang, Chun-Yan,Zheng, Hai-Ying,Jing-Qin,Wang, Jian,Huang, Fen-Xiang,Yang, Bing-Zhuang,Liang, Xiang-Wei. 2013

[11]Effect of 5-aza-2 '-deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos. Zhao, Xue-Ming,Ren, Jing-Jing,Du, Wei-Hua,Hao, Hai-Sheng,Wang, Dong,Liu, Yan,Qin, Tong,Zhu, Hua-Bin. 2012

[12]Seed traits evaluation from long-term selection of kernel oil concentration in a high-oil maize population KYHO. Wang Hong-Wu,Hu Hai-Xiao,Song Tong-Ming,Chen Shao-Jiang,Wang Hong-Wu. 2012

[13]Melatonin inhibits paraquat-induced cell death in bovine preimplantation embryos. Pang, Yun-Wei,Sun, Ye-Qing,Sun, Wei-Jun,Du, Wei-Hua,Hao, Hai-Sheng,Zhao, Shan-Jiang,Zhu, Hua-Bin. 2016

[14]Stage-Specific developmental gene expression of goat preimplantation embryos produced in vitro. Li, Yong-Jun,Zhang, Li,Li, Yong-Jun,Ao, Hong,Sun, Gui-Jin,Sun, Gui-Jin.

[15]Establishment of Eimeria tenella (local isolate) in chicken embryos. Jiang, L.,Zhao, Q.,Zhu, S.,Han, H.,Dong, H.,Huang, B..

[16]Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines. Zhang, Chun-Lai,Chen, Dong-Fang,Kubalakova, Marie,Zhang, Jian,Scott, Nigel W.,Elliott, Malcolm C.,Slater, Adrian,Zhang, Chun-Lai,Chen, Dong-Fang,Kubalakova, Marie,Zhang, Jian.

[17]Differentially expressed genes in white egg 2 mutant of silkworm, Bombyx mori, at early embryo development stages. Zhang, Guozheng,Lou, Chengfu,Zhang, Guozheng,Wei, Yadong,Xia, Dingguo,Zhang, Yeshun,Deng, Bo,Liu, Ziyu,Zhang, Jiekui,Zhang, Guozheng,Wei, Yadong,Xia, Dingguo,Zhang, Yeshun. 2011

[18]Changes of proteome and phosphoproteome trigger embryo-larva transition of honeybee worker (Apis mellifera ligustica). Gala, Alemayehu,Fang, Yu,Woltedji, Dereje,Zhang, Lan,Han, Bin,Feng, Mao,Li, Jianke.

[19]Efficiency of in vitro embryo production of yak (Bos grunniens) cultured in different maturation and culture conditions. Guo, Xian,Ding, Xuezhi,Pei, Jie,Bao, Pengjia,Liang, Chunnian,Chu, Min,Yan, Ping,Guo, Xian,Ding, Xuezhi,Pei, Jie,Bao, Pengjia,Liang, Chunnian,Chu, Min,Yan, Ping. 2012

[20]Identification and characterization of the zinc-regulated transporters, iron-regulated transporter-like protein (ZIP) gene family in maize. Li, Suzhen,Huang, Yaqun,Zhu, Liying,Zhao, Yongfeng,Guo, Jinjie,Chen, Jingtang,Li, Suzhen,Zhou, Xiaojin,Zhang, Shaojun,Chen, Rumei. 2013

作者其他论文 更多>>