数字农科院2.0

Butyrate Consolidates the Positive Effects of the Mannose–Cetobacterium Axis in Improving Liver and Intestinal Health of High-Fat Diet–Fed Zebrafish

文献类型： 外文期刊

作者： Delong Meng;Xiaoying Zhu;Tsegay Teame;Benjamin Earl Niemann;Ran Zhang;Hongwei Yang;Chao Ran;Yuanyuan Yao;Qianwen Ding;Yalin Yang;Zhen Zhang;Zhigang Zhou

作者机构：

关键词： Cetobacterium;intestinal health;liver steatosis;mannose;tributyrin;zebrafish

期刊名称： Journal of Nutrition

ISSN： 0022-3166

年卷期： 2025 年 156 卷 2 期

页码：

收录情况： SCIE（2025版）

摘要： Background: Intestinal and liver damage induced by a high-fat diet (HFD) is a major problem in farmed fish. Cetobacterium somerae is a native beneficial bacterium to many fish species, including zebrafish, a model animal for nutrition studies of economically important fish species. Objectives: The aim of this study was to investigate the mechanisms how mannose and tributyrin affect the growth of C. somerae and its role in ameliorating HFD-induced liver and intestinal damage, and gut microbiota dysbiosis of zebrafish. Methods: One-month-old zebrafish were fed for 4 wk with Control (5.77% fat), HFD (14.64% fat), or HFD supplemented with 1.0% mannose (experiment 1) and 0.1% tributyrin (experiment 2). Then, a combination treatment of mannose and tributyrin was tested with an HFD and compared their effects with the previous single additive groups (experiment 3). Finally, the effects of Cetobacterium and Plesiomonas on alleviating HFD-induced liver steatosis were investigated using germ-free (GF) zebrafish (experiment 4). Results: When Cetobacterium abundance was low in the gut of HFD-fed zebrafish, the prebiotic mannose could not improve the growth of Cetobacterium to elicit any beneficial effects. Supplementation with tributyrin created an appropriate anaerobic gut environment for Cetobacterium growth by upregulating the expression of the hif1a gene by 86.4% (P < 0.05). The combination of mannose and tributyrin increased the abundance of Cetobacterium by 309.8% compared with the HFD group. In addition, the combination treatment reduced triacylglycerol, Alanine aminotransferase, and aspartic aminotransferase levels (0.61-, 0.46-, and 0.55-fold, respectively; P < 0.05) and improved HFD-induced liver damage. Furthermore, intestinal damage was improved as verified with a lower level of serum LPS (0.59-fold, P < 0.05). After transferring Cetobacterium into GF zebrafish, the liver lipid accumulation caused by HFD was reduced by 10.9% (P < 0.05). Conclusions: The combination of mannose and tributyrin enhanced the growth of Cetobacterium and its benefits in improving HFD-induced liver and intestinal damage.

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