数字农科院2.0

A novel Tembusu virus isolated from goslings in China form a new subgenotype 2.1.1

文献类型： 外文期刊

作者： Zhu, Yingqi;Hu, Zengjin;Lv, Xuan;Huang, Rong;Gu, Xiangxue;Zhang, Chong;Zhang, Miao;Wei, Juanwen;Wu, Qiong;Li, Jiaming;Zhang, Ruichen;Cao, Shoulin;Yin, Dongdong;Wang, Bei;Liu, Guangqing;Wang, Guijun

作者机构：

关键词： DTMUV/goose/China/2019/AQ-19;genetic variation;high virulence;new cluster

期刊名称： TRANSBOUNDARY AND EMERGING DISEASES

ISSN： 1865-1674

年卷期： 2021 年

页码：

收录情况： JCR（2021版）

摘要： Since 2010, several duck Tembusu viruses (DTMUVs) have been isolated from infected ducks in China, and these virus strains have undergone extensive variation over the years. Although the infection rate is high, the mortality rate is usually relatively low-similar to 5%-30%; however, since fall 2019, an infectious disease similar to DTMUV infection but with a high mortality rate of similar to 50% in goslings has been prevalent in Anhui Province, China. The present study identified a new Tembusu virus, designated DTMUV/Goose/China/2019/AQ-19 (AQ-19), that is believed to be responsible for the noticeably high mortality in goslings. To investigate the genetic variation of this strain, its entire genome was sequenced and analysed for specific variations, and goslings and mice were challenged with the isolated virus to investigate its pathogenicity. The AQ-19 genome shared only 94.3%-96.9% and 90.9% nucleotide identity with other Chinese and Malaysian DTMUVs, respectively; however, AQ-19 has high homology with Thailand DTMUVs (97.2%98.1% nucleotide identity). Phylogenetic analysis of the E gene revealed that AQ-19 and most of Thailand DTMUVs form a branch separate from any of the previously reported DTMUV strains in China. After the challenge, some goslings and mice showed typical clinical signs of DTMUV, particularly severe neurological dysfunction. AQ-19 has high virulence in goslings and mice, resulting in 60% and 70% mortality through intramuscular and intracerebral routes, respectively. Pathological examination revealed severe histological lesions in the brain and liver of the infected goslings and mice. Taken together, these results demonstrated the emergence of a novel Tembusu virus with high virulence circulating in goslings in China for the first time, and our findings highlight the high genetic diversity of DTMUVs in China. Further study of the pathogenicity and host range of this novel Tembusu virus is particularly important.

分类号：

  • 相关文献

[1]Intraspecific and interspecific variation in Setaria revealed by RAPD analysis. Li, Y,Jia, JZ,Wang, Y,Wu, SZ. 1998

[2]Sequence variability in four mitochondrial genes among rabbit pinworm (Passalurus ambiguus) isolates from different localities in China. Sheng, Li,Zou, Feng-Cai,Zhu, Xing-Quan,Sheng, Li,Zhu, Xing-Quan,Cui, Ping,Fang, Su-Fang,Lin, Rui-Qing. 2015

[3]Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China. Chen, Jianfei,Liu, Xiaozhen,Shi, Da,Shi, Hongyan,Zhang, Xin,Li, Changlong,Chi, Yanbin,Feng, Li. 2013

[4]Genetic variation and population structure of the mushroom Pleurotus ferulae in China inferred from nuclear DNA analysis. Zhao Meng-ran,Huang Chen-yang,Wu Xiang-li,Chen Qiang,Qu Ji-bin,Gao Wei,Zhang Jin-xia,Li Yan-chun. 2016

[5]Experimental infection and comparative genomic analysis of a highly pathogenic PRRSV-HBR strain at different passage levels. Wei, Yanwu,Li, Shengbin,Huang, Liping,Tang, Qinghai,Liu, Jianbo,Liu, Dan,Wang, Yiping,Wu, Hongli,Liu, Changming. 2013

[6]Population Genetic Analysis of the Rice Stem Borer, Chilo suppressalis, in the South China. Hou Mao-lin,Liu Yu-di,Wu Yu-chun,Liu Gui-qin. 2013

[7]Structural and evolutionary divergence of aquaporins in parasites. Ni, Zi-Xin,Cui, Jian-Min,Zhang, Nian-Zhang,Fu, Bao-Quan,Fu, Bao-Quan.

[8]Development of a SCAR marker by inter-simple sequence repeat for diagnosis of dwarf bunt of wheat and detection of Tilletia controversa Kuhn. Gao, L.,Chen, W. Q.,Liu, T. G..

[9]Low-nitrogen stress tolerance and nitrogen agronomic efficiency among maize inbreds: comparison of multiple indices and evaluation of genetic variation. Li, Xinhai,Li, Mingshun,Zhang, Degui,Hao, Zhuanfang,Weng, Jianfeng,Xu, Yunbi,Bai, Li,Zhang, Shihuang,Xie, Chuanxiao,Wu, Yongshen,Liu, Wenguo,Xu, Yunbi,Xu, Yunbi.

[10]Genetic Variation of High Molecular Weight Glutenin Subunits in Wheat Accessions in China. Guo, Xiaomin,Li, Hongqin,Xiang, Jishan,Xu, Xin,Liu, Weihua,Gao, Ainong,Yang, Xinming,Li, Xiuquan,Li, Lihui,Wang, Ruihui.

[11]Genetic variation in V gene of class II Newcastle disease virus. Hao, Huafang,Liu, Peng,Ren, Shanhui,Gao, Xiaolong,Wang, Yanping,Wang, Xinglong,Zhang, Shixia,Yang, Zengqi,Chen, Shengli.

[12]A novel statistical method for assessing SSR variation in autotetraploid alfalfa (Medicago sativa L.). Liu, Zhi-Peng,Liu, Gong-She,Yang, Qing-Chuan.

[13]Genetic diversity among a founder parent and widely grown wheat cultivars derived from the same origin based on morphological traits and microsatellite markers. Li, X. J.,Xu, X.,Yang, X. M.,Li, X. Q.,Liu, W. H.,Gao, A. N.,Li, L. H.,Li, X. J.,Xu, X..

[14]Advances in molecular identification, taxonomy, genetic variation and diagnosis of Toxocara spp.. Chen, Jia,Zhou, Dong-Hui,Xu, Min-Jun,Huang, Si-Yang,Li, Ming-Wei,Zhu, Xing-Quan,Chen, Jia,Nisbet, Alasdair J.,Li, Ming-Wei,Wang, Chun-Ren,Zhu, Xing-Quan,Zhu, Xing-Quan.

[15]Discovery, evaluation and distribution of haplotypes of the wheat Ppd-D1 gene. Guo, Zhiai,Song, Yanxia,Zhou, Ronghua,Jia, Jizeng,Guo, Zhiai,Ren, Zhenglong.

[16]Phylogenetic analysis of the haemagglutinin gene of canine distemper virus strains detected from breeding foxes, raccoon dogs and minks in China. Zhao, Jian-Jun,Yan, Xi-Jun,Chai, Xiu-Li,Luo, Guo-Liang,Zhang, Hai-Ling,Gao, Han,Bai, Xue,Zhang, Lei,Chen, Tao,Xu, Lei,Zhao, Chun-Fei,Wang, Feng-Xue,Shao, Xi-Qun,Wu, Wei,Cheng, Shi-Peng,Martella, Vito,Liu, Ying-Xue.

[17]AFLP analyses of genetic variation of Eupatorium adenophorum (Asteraceae) populations in China. Huang, W. K.,Wan, F. H.,Guo, J. Y.,Peng, D. L.,Huang, W. K.,Gao, B. D.,Xie, B. Y..

[18]Exploitation of Malus EST-SSRs and the utility in evaluation of genetic diversity in Malus and Pyrus. Yao, Lihua,Zheng, Xiaoyan,Cai, Danying,Teng, Yuanwen,Yao, Lihua,Zheng, Xiaoyan,Cai, Danying,Teng, Yuanwen,Gao, Yuan,Wang, Kun,Cao, Yufen.

[19]Allelic diversity and geographical pattern at soybean Kunitz trypsin inhibitor single locus in Chinese wild soybean (Glycine soja Sieb. & Zucc.). Wang, K. J.,Li, X. H.,Takahata, Y.,Yamashita, T..

[20]Genetic diversity and differentiation of Ankole cattle populations in Uganda inferred from microsatellite data. Kugonza, D. R.,Mpairwe, D.,Kiwuwa, G. H.,Kugonza, D. R.,Jianlin, H.,Okeyo, A. M.,Hanotte, O.,Jianlin, H.,Nabasirye, M..

作者其他论文 更多>>