数字农科院2.0

Over-Expression of Tomato GDP-Mannose Pyrophosphorylase (GMPase) in Potato Increases Ascorbate Content and Delays Plant Senescence

文献类型： 外文期刊

作者： Yu Xian-chang;Lin Ling-ling;Shi Qing-hua;Qin Ai-guo;Wang Hua-sen

作者机构：

关键词： potato;GDP-mannose pyrophosphorylase;ascorbate;senescence

期刊名称： AGRICULTURAL SCIENCES IN CHINA

ISSN： 1671-2927

年卷期： 2011 年 10 卷 4 期

页码：

收录情况： SCI

摘要： GDP-D-mannose pyrophosphorylase (GMPase) catalyses the synthesis of GDP-D-mannose and represents the first committed step in the synthesis of ascorbate. In the present study, the GMPase gene of tomato was introduced into potato by Agrobacterium-mediated transformation. Two transgenic lines with higher GMPase expression were selected using qPCR and protein blot analyses. The results showed that the content of L-ascorbic acid (AsA) and the ratio of AsA/DHA (dehydroascorbate) significantly increased in both leaves and tubers of transgenic potato plants. Both pigment content and photosynthetic rate were much higher in transgenic plants than in wild-type plants. Transgenic plants showed a distinguishable change in phenotype from the wild-type plants. Furthermore, transgenic plants showed delayed senescence.

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