数字农科院2.0

Determination of Organophosphorus Pesticides Based on Biotin-Avidin Enzyme Linked Immunosorbent Assay

文献类型： 外文期刊

作者： Wang Lei;Zhang Li-Jie;Lue Wei;Han Shi-He;Zhang Fu-Kai;Pan Jia-Rong;Han Shi-He

作者机构：

关键词： Pesticides;O,O-Diethyl organophosphorus;Biotin-avidin amplified enzyme linked immunosorbent assay

期刊名称： CHINESE JOURNAL OF ANALYTICAL CHEMISTRY

ISSN： 0253-3820

年卷期： 2011 年 39 卷 3 期

页码：

收录情况： SCI

摘要： A multienzyme system was generated by the specific reaction between biotin and avidin. Based on the system, a biotin-avidin amplified enzyme-linked immunosorbent assay ( BA-ELISA) method was developed and optimized for the determination of O,O-diethyl organophosphorus pesticides. Under the optimized experiment conditions, a good linear relationship between the concentrations of O, O-diethyl organophosphorus pesticides and inhibition ratio in the range of 0. 1-1. 0 X 10(4)mu g/L was obtained. The regression equation for the determination of diethylphosphonoacetic acid (DPA) was y=0. 1168 1gC(DPA) +0. 6259 (r = 0. 9889), 1050 and the detection limit were 34 and 0.0248 mu g/L, respectively. Compared with the ELISA, the sensitivity and detecting range were significantly improved while the cross-reaction remained unchanged. The recovery of the spiked samples was in the range from 70. 1% to 117. 8%, basically meeting the requirement of trace analysis. (O,O-diethyl organophosphorus pesticides.

分类号：

  • 相关文献

[1]Effective duration of pesticide-induced susceptibility of rice to brown planthopper (Nilaparvata lugens Stal, Homoptera : Delphacidae), and physiological and biochemical changes in rice plants following pesticide application. Wu, JC,Qiu, HM,Yang, GQ,Liu, JL,Liu, GJ,Wilkins, RM. 2004

[2]Opportunities of Knowledge and Technology Exchange between China and Europe to Solve Common Groundwater Management Problems Related to Agriculture. Wilko, Schweers. 2008

[3]Simultaneous Determination of 70 Pesticide Residues in Coffees by Gas Chromatography-Mass Spectrometry. Yang, Xin,Wang, Jing,Yang, Xin,Zhang, Hua,Xu, Dechang,Qiu, Jianwei,Wang, ZhenYu,Cao, WeiQiang. 2010

[4]Study on multiple pesticides by High Performance Liquid Chromatography-Mass Spectrometry using modified QuEChERS method - A case study on CHM. Dong, Yiwei,Bai, Wei,Li, Yuzhong,Xu, Chunying,Fang, Fuli,Guo, Zhicheng. 2012

[5]Multiresidue determination of 114 multiclass pesticides in flue-cured tobacco by solid-phase extraction coupled with gas chromatography and tandem mass spectrometry. Cao, Jianmin,Sun, Na,Yu, Weisong,Pang, Xueli,Lin, Yingnan,Kong, Fanyu,Qiu, Jun.

[6]Novel acetylcholinesterase inhibitors: Synthesis and structure-activity relationships of phthalimide alkyloxyphenyl N,N-dimethylcarbamate derivatives. Yang, Guoquan,Mei, Xiangdong,Yuan, Huizhu,Ning, Jun,Zhao, Qianfei.

[7]Multiresidue method for determination of 88 pesticides in berry fruits using solid-phase extraction and gas chromatography-mass spectrometry: Determination of 88 pesticides in berries using SPE and GC-MS. Yang, X.,Zhang, H.,Zhang, Y. C.,Dong, A. J.,Zhao, H. T.,Cui, J.,Liu, Y.,Yang, X.,Wang, J.,Sun, C. H..

[8]Simultaneous determination of 118 pesticide residues in Chinese teas by gas chromatography-mass spectrometry. Yang, Xin,Xu, De Chang,Qiu, Jian Wei,Zhang, Hua,Zhang, Ying Chun,Dong, Ai Jun,Ma, Ying,Wang, Jing,Wang, Jing.

[9]Evaluation of surface-enhanced Raman scattering detection using a handheld and a bench-top Raman spectrometer: A comparative study. Zheng, Jinkai,Pang, Shintaro,He, Lili,Zheng, Jinkai,Labuza, Theodore P..

[10]Transfer Rates of 19 Typical Pesticides and the Relationship with Their Physicochemical Property. Chen, Hongping,Pan, Rong,Zhang, Minglu,Liu, Xin,Lu, Chengyin,Chen, Hongping,Pan, Meiling,Liu, Xin,Lu, Chengyin,Chen, Hongping,Liu, Xin,Lu, Chengyin.

[11]Pesticide productivity, host-plant resistance and productivity in China. Widawsky, D,Rozelle, S,Jin, SQ,Huang, JK. 1998

[12]Stereoselective determination of benalaxyl in plasma by chiral high-performance liquid chromatography with diode array detector and application to pharmacokinetic study in rabbits. Wang, Qiuxia,Zhu, Wentao,Jia, Guifang,Wang, Xinquan,Zhou, Zhiqiang.

[13]Enantioselective effects of metalaxyl on soil enzyme activity. Yue, Heng,Fang, Song,Zhang, Yizhi,Ning, Yang,Yu, Weisong,Kong, Fanyu,Qiu, Jun,Yue, Heng,Fang, Song,Zhang, Yizhi,Ning, Yang,Yu, Weisong,Kong, Fanyu,Qiu, Jun.

[14]Comparison of different cleanup procedures for oil crops based on the development of a trace analytical method for the determination of pyraclostrobin and epoxiconazole. Dong, Fengshou,Xu, Jun,Liu, Xingang,Cheng, Youpu,Chen, Zenglong,Liu, Na,Chen, Xixi,Tao, Yan,Zheng, Yongquan.

[15]Field-Testing of Synthetic Herbivore-Induced Plant Volatiles as Attractants for Beneficial Insects. Zhang, Yongjun,Wu, Kongming,Guo, Yu Yuan,Yu, Huilin.

[16]Lethal and Sublethal Effects of Endosulfan on Apolygus lucorum (Hemiptera: Miridae). Lu, Yanhui,Wu, Kongming,Liu, Yangqing,Xue, Fangsen,Wyckhuys, Kris A. G..

[17]Simultaneous Determination of 69 Pesticide Residues in Coffee by Gas Chromatography-Mass Spectrometry. Yang, Xin,Xu, D. C.,Qiu, J. W.,Ma, Ying,Cui, J.,Yang, Xin,Wang, Jing. 2011

[18]Ubi1 intron-mediated enhancement of the expression of Bt cry1Ah gene in transgenic maize (Zea mays L.). Lang Zhihong,Huang DaFang,Zhang Jie,He KangLai,Song FuPing.

[19]Residue behaviour of six pesticides in button crimini during home canning. Liu, Xingang,Dong, Fengshou,Xu, Jun,Li, Yuanbo,Zheng, Yongquan,Gu, Xiaojun,Kong, Zhiqiang. 2014

[20]The single and joint toxicity effects of chlorpyrifos and beta-cypermethrin in zebrafish (Danio rerio) early life stages. Zhang, Jiayu,Liu, Lili,Ren, Lei,Feng, Weimin,Lv, Peng,Wu, Wei,Yan, Yanchun.

作者其他论文 更多>>