数字农科院2.0

Expression and Activity Analysis of Non-Structural Protein 2 (NS2) of Bombyx mori Densovirus Zhenjiang Strain

文献类型： 外文期刊

作者： Zhao Pan;Tang Shun-ming;Guo Xi-jie;Tang Shun-ming;Liu Ting;Qin Guang-xing;Guo Xi-jie

作者机构：

关键词： Bombyx mori;densovirus;non-structure protein;expression;activity

期刊名称： AGRICULTURAL SCIENCES IN CHINA

ISSN： 1671-2927

年卷期： 2010 年 9 卷 12 期

页码：

收录情况： SCI

摘要： The gene of the non-structure protein 2 (NS2) was cloned by PCR from the genome of Bombyx mori densovirus Zhenjiang strain (BmDNV-Z), inserted into prokaryotic expression vector pET28a to construct recombinant plasmid pET28a-NS2 and then expressed in bacteria Escherichia coli BL21 (DE3) The expressed recombinant protein was identified by SDS-PAGE and Western blot analysis Then, the recombinant protein was purified by Ni-NTA column, renatured and tested for enzyme activities The purified NS2 protein exhibited a helicase activity unwinding double-stranded DNA substrates into single-strand primers, and higher unwinding activity to polarity substrate Similarly, the purified NS2 protein possessed an ATPase activity and its enzyme activity was 0 276 mu mol mu g (1) h (1) in this study The results indicated that the non-structure protein which encoded by the gene of BmDNV-Z NS2 possesses the biological activities of helicase and ATPase, and the helicase prefers to polarity substrates Based on these results, it is speculated that the gene of BmDNV-Z NS2 plays an important role in the viral DNA replication

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