数字农科院2.0

Expression pattern and core region analysis of AtMPK3 promoter in response to environmental stresses

文献类型： 外文期刊

作者： Gao Fei;Su Qi;Fan YunLiu;Wang Lei;Gao Fei

作者机构：

关键词： Arabidopsis thaliana;AtMPK3;promoter;stress

期刊名称： SCIENCE CHINA-LIFE SCIENCES

ISSN： 1674-7305

年卷期： 2010 年 53 卷 11 期

页码：

收录情况： SCI

摘要： The protein kinase AtMPK3, a component of the MAP kinase cascade, plays an important role in stress signal transduction in plant cells. To clarify how AtMPK3 is regulated at the transcriptional level in response to various environmental factors, the 1016-bp promoter sequence upstream of the transcription start site of the AtMPK3 gene was isolated. Analyses of the promoter sequence using plant promoter databases revealed that the AtMPK3 promoter contains many potential cis-acting elements involved in environmental stress responses. We constructed four deletion mutants of the AtMPK3 promoter, and introduced the intact and truncated promoter sequences fused to the beta-glucuronidase (GUS) gene into Arabidopsis. GUS histochemical staining and quantitative fluorometric GUS assays were performed to visualize and compare the expression patterns in response to different environmental stimuli. The region between -188 and -62 upstream of the transcription start site was identified as the essential DNA sequence of the AtMPK3 promoter for responses to drought, high salinity, low temperature, and wounding. These results advance our understanding of the molecular mechanisms controlling AtMPK3 expression in response to different environmental stimuli.

分类号：

  • 相关文献

[1]GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots. Chen, Li,Jiang, Bingjun,Wu, Cunxiang,Sun, Shi,Hou, Wensheng,Han, Tianfu. 2014

[2]Isolation and Functional Analysis of Convolvulus arvensis EPSPS Promoter. Wang, Guirong,Huang, Hongjuan,Wei, Shouhui,Zhou, Xinxin,Chen, Jinyi,Chen, Jingchao,Zhang, Chaoxian.

[3]Genome-Wide Characterization of the Phosphofructokinase Gene Family in Arabidopsis thaliana and Functional Analysis of AtPFK2 in Stress Tolerance. Siyu Liu,Jiheng Gou,Yunni Tang,Yunxiao Wei,Rui Zhang. 2025

[4]EIAV基因转移载体启动子的比较. 韩凌霞,李亚明,曲连东,司昌德,姜骞,刘家森. 2006

[5]拟南芥AtMPK3启动子应逆境信号的表模和必需区域分析. 高飞,苏琦,范云六,王磊. 2010

[6]拟南芥AtMPK3启动子应逆境信号的表模和必需区域分析. 高飞,苏琦,范云六,王磊. 2010

[7]拟南芥AtMPK3启动子应答逆境信号的表达模式和必需区域分析. 高飞,苏琦,范云六,王磊. 2010

[8]Characterization of a Maize Wip1 Promoter in Transgenic Plants. Zhang, Shengxue,Liu, Yan,Wang, Xiaoqing,Liu, Yunjun,Wang, Guoying,Lian, Yun. 2013

[9]Isolation and characterization of Brittle2 promoter from Zea Mays and its comparison with Ze19 promoter in transgenic tobacco plants. Chen, Xiaoping,Wang, Zhangying,Wang, Jianhua,Wang, Maoyan,Zhao, Li,Wang, Guoying. 2007

[10]Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco. Gu, Riliang,Zhao, Li,Zhang, Ying,Chen, Xiaoping,Bao, Juan,Zhao, Jinfeng,Wang, Zhangying,Fu, Junjie,Liu, Tingsong,Wang, Jianhua,Wang, Guoying. 2006

[11]Analysis of promoters of microRNAs from a Glycine max degradome library. Han, Yi-qiang,Gao, Ya-mei,Hu, Zheng,Zheng, Dian-feng. 2014

[12]The characterization of GmTIP, a root-specific gene from soybean, and the expression analysis of its promoter. Chen, Li,Jiang, Bingjun,Wu, Cunxiang,Sun, Shi,Hou, Wensheng,Han, Tianfu.

[13]Transcriptional regulation of the gene for prothoracicotropic hormone in the silkworm, Bombyx mori. Wei, Zhao-Jun,Yu, Miao,Hong, Gui-Yun,Jiang, Shao-Tong,Tang, Shun-Ming,Yi, Yong-Zhu.

[14]Subcellular localization, expression patterns, SNPs and association analyses of the porcine HUMMLC2B gene. Wang, Huan L.,Wang, Heng,Zhu, Zheng M.,Wang, Chen F.,Zhu, Meng J.,Mo, De L.,Yang, Shu L.,Li, Kui.

[15]Isolation, Characterization and Promoter Analysis of Cell Wall Invertase Gene SoCIN1 from Sugarcane (Saccharum spp.). Wang, Ai-Qin,Huang, Jing-Li,Yang, Li-Tao,Yang, Li-Tao,Li, Yang-Rui,Yang, Li-Tao,Li, Yang-Rui. 2015

[16]Comparison of six promoters for transient expression of luciferase reporter gene in cultured Bombyx mori cells (BmN). Huang, Yong,Wang, Jian Ping,Huang, Yong,Song, Fei,Wang, Xin,Shen, Xing Jia.

[17]Cloning and function analysis of an alfalfa (Medicago sativa L.) zinc finger protein promoter MsZPP. Yang, Qingchuan,Kang, Junmei,Zhang, Tiejun,Sun, Yan,Gruber, Margaret Yvonne,Fang, Feng.

[18]Characterization of the NLRC5 promoter in chicken: SNPs, regulatory elements and CpG islands. Guo, X. M.,Chang, G. B.,Xu, L.,Bi, Y. L.,Zhang, Y.,Zhu, P. F.,Wu, Y.,Chen, G. H.,Liu, X. P.,Wang, H. Z..

[19]Molecular cloning and characterization of the promoter region of the porcine apolipoprotein E gene. Xia, Jihan,Hu, Bingjun,Mu, Yulian,Xin, Leilei,Yang, Shulin,Li, Kui.

[20]GENOMIC ANALYSIS OF SILKWORM microRNA PROMOTERS AND CLUSTERS. Huang, Yong,Shen, Xing Jia,Huang, Jin Shan,Tang, Shun Ming,Huang, Yong,Shen, Xing Jia,Huang, Jin Shan,Tang, Shun Ming,Zou, Quan.

作者其他论文 更多>>