数字农科院2.0

Merozoite proteins from Babesia sp BQ1 (Lintan) as potential antigens for serodiagnosis by ELISA

文献类型： 外文期刊

作者： Guan, G. Q.;Chauvin, A.;Moreau, E.;Guan, G. Q.;Luo, J. X.;Yin, H.;Rogniaux, H.

作者机构：

关键词： Babesia sp BQ1 (Lintan);antigen;ELISA;merozoite;cross-reaction;antibody kinetics;prevalence

期刊名称： PARASITOLOGY

ISSN： 0031-1820

年卷期： 2010 年 137 卷 6 期

页码：

收录情况： SCI

摘要： Babesia sp BQ1 (Lintan) is Babesia isolated from sheep infested with Haemaphysalis qinghatensis in China, and is closely related to B motasi, based on the I SS rRNA gene sequence In the present study, an ELISA was des eloped with merozoite antigens of Babesia sp BQ1 (Liman) (BQMA) purified horn in vino culture When the positive threshold was chosen as 30% of the antibodies rate, evaluated with 198 negative sera, the specificity was 95 5% Except for Babasia sp Tianzhu, there was no cross-reaction between BQMA and positive sera from Babesia sp BQI (Ningxian)-, Babesia sp Babesia sp Xinjiang-, Theileria luwenshum-, T utlenbergi-. or Anaplasma ovis-infected sheep, which are the dominant haemoparasites of small ruminants in China Specific antibodies against Babesia sp BQ1 I (Liman) were produced 1 or 2 weeks post-Infection and a high level of antibodies persisted for more than 8 months in experimentally infected sheep This ELISA was tested on 974 sera collected from field-grazing sheep in 3 counties of Gansu province, northwestern China to evaluate the seroprevalence of Babesia sp 1301 (Lintan) Infection and the average positive rate was 66 84% The feasibility of increasing the specificity of this BQMA-based ELISA, by using some BQMA antigens for serodiagnosis is discussed

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