数字农科院2.0

Effects on In vitro Replication of Equine Infectious Anemia Virus Attenuated Vaccine Strain With Truncated Mutation in The Transmembrane Protein

文献类型： 外文期刊

作者： Jiang Cheng-Gang;Liu Di;Jiang Cheng-Gang;Ma Jian;Gao Xu;Lin Yue-Zhi;Zhao Li-Ping;Zhou Jian-Hua;Hua Yu-Ping;Gao Xu

作者机构：

关键词： equine infectious anemia virus;gp45 transmembrane protein;truncated mutation;replication;apoptosis

期刊名称： PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS

ISSN： 1000-3282

年卷期： 2010 年 37 卷 3 期

页码：

收录情况： SCI

摘要： Equine infectious anemia virus (EIAV) vaccine is the first successfully applied lentiviral vaccine, but its mechanism on inducing protective immunity is not clear. Previous studies found that the EIAV vaccine strain EIAV(FDDV12) transmembrane protein (gp45) had a high-frequent translational terminating mutation at the site of 261W, resulting in a truncation of 154 amino acid residues at the C-terminus. To explore the biological meaning of the gp45 truncation, a gp45-truncated molecular clone was constructed by using an infectious clone of EIAV(FDDV12) as the backbone. Replications features of the gp45-truncated EIAV and its prototype virus were analyzed and compared in cultivated monocyte-derived macrophages (MDM) of equine and donkey and fetal donkey dermal cells (FDD). Results showed that the replication capacity of the gp45-truncated EIAV in equine and donkey MDMs was significantly decreased compared to the untruncated gp45 EIAV(P < 0.01), especially in the horse macrophages. In contrast, the truncated EIAV replicated significantly faster than the untruncated EIAV in FDD cells (P < 0.01). In addition, the reduced replication of the gp45-truncated EIAV in equine MDM led to a significant decline of cytotoxicity of the host cells when compared with the gp45 untruncated EIAV (P < 0.05). These results suggest that the truncation of the 154-residue C-terminus of the EIAV gp45 glycoprotein was an adaptation to the attenuation of the EIAV vaccine strain in FDD cells. This truncated mutation reduces the replication of the vaccine strain in macrophages, the primary in vivo target cell of EIAV, which leads to a further attenuation of the vaccine.

分类号：

  • 相关文献

[1]猪繁殖与呼吸综合征病毒感染和复制机制及其毒力研究进展AdvancesinUnderstandingoftheInfection/ReplicationMechanismsandVirulenceDeterminantsofthePorcineReproductiveandRespiratorySyndromeVirus. 刘杏,王凤雪,温永俊*LIUXing,WANGFengxue,WENYongjun*. 2015

[2]Evolutionary Patterns of the Proviral gp90 V3 to V5 Regions of Equine Infectious Anemia Virus Associated with Immune Selection in Progressors and Nonprogressors. Yuan Xiu-fang,Zhou Tao,Hou Shao-hua,Tu Ya-bin,Peng Jin-mei,Wen Jian-xin,Qiu Hua-ji,Wu Dong-lai,Tong Guang-zhi,Yuan Xiu-fang,Tong Guang-zhi. 2011

[3]A Unique Evolution of the S2 Gene of Equine Infectious Anemia Virus in Hosts Correlated with Particular Infection Statuses. Wang, Xue-Feng,Wang, Shuai,Liu, Qiang,Lin, Yue-Zhi,Du, Cheng,Tang, Yan-Dong,Na, Lei,Wang, Xiaojun,Zhou, Jian-Hua,Zhou, Jian-Hua. 2014

[4]Unique evolution characteristics of the envelope protein of EIAV(LN40), a virulent strain of equine infectious anemia virus. Wang, Xuefeng,Wang, Shuai,Lin, Yuezhi,Jiang, Chenggang,Ma, Jian,Zhao, Liping,Lv, Xiaoling,Shen, Rongxian,Zhou, Jianhua,Wang, Xuefeng,Wang, Fenglong. 2011

[5]Equine schlafen 11 restricts the production of equine infectious anemia virus via a codon usage-dependent mechanism. Lin, Yue-Zhi,Sun, Liu-Ke,Zhu, Dan-Tong,Hu, Zhe,Wang, Xue-Feng,Du, Cheng,Wang, Xiao-Jun,Zhou, Jian-Hua,Zhu, Dan-Tong,Wang, Yu-Hong,Zhou, Jian-Hua.

[6]Inhibition of virus replication and induction of human tetherin gene expression by equine IFN-alpha 1. Hu, Zhe,Wu, Xingliang,Ge, Jinying,Wang, Xiaojun.

[7]Infection with equine infectious anemia virus vaccine strain EIAV(DLV121) causes no visible histopathological lesions in target organs in association with restricted viral replication and unique cytokine response. Liu, Qiang,Ma, Jian,Wang, Xue-Feng,Xiao, Fei,Li, Li-Jia,Zhang, Jiao-Er,Lin, Yue-Zhi,Du, Cheng,He, Xi-Jun,Wang, Xiaojun,Zhou, Jian-Hua,Zhou, Jian-Hua.

[8]C-terminal truncation of the transmembrane protein of an attenuated lentiviral vaccine alters its in vitro but not in vivo replication and weakens its potential pathogenicity. Jiang, Cheng-Gang,Gao, Xu,Ma, Jian,Lin, Yue-Zhi,Wang, Xue-Feng,Zhao, Li-Ping,Zhou, Jian-Hua,Jiang, Cheng-Gang,Liu, Di,Hua, Yue-Ping,Gao, Xu. 2011

[9]Newcastle disease virus induces stable formation of bona fide stress granules to facilitate viral replication through manipulating host protein translation. Sun, Yingjie,Dong, Luna,Yu, Shengqing,Wang, Xiaoxu,Zheng, Hang,Zhang, Pin,Meng, Chunchun,Zhan, Yuan,Tan, Lei,Song, Cuiping,Qiu, Xusheng,Liao, Ying,Ding, Chan,Dong, Luna,Zheng, Hang,Wang, Xiaoxu,Wang, Guijun,Ding, Chan.

[10]Mutational analysis of the SDD sequence motif of a PRRSV RNA-dependent RNA polymerase. Zhou Yan,Zheng HaiHong,Gao Fei,Tian DeBin,Yuan ShiShan. 2011

[11]Endoplasmic reticulum stress-mediated autophagy contributes to bluetongue virus infection via the PERK-eIF2 alpha pathway. Lv, Shuang,Sun, En-Cheng,Xu, Qing-Yuan,Zhang, Ji-Kai,Wu, Dong-Lai.

[12]Effect of ORF119 gene deletion on the replication and virulence of orf virus. Qiao, J.,Yang, H. B.,Peng, Y. L.,Meng, Q. L.,Chen, C.,Ma, Y.,Xie, K.,Liu, T. L.,Chen, C. F.,Cai, X. P..

[13]Cysteine residues of the porcine reproductive and respiratory syndrome virus ORF5a protein are not essential for virus viability. Sun, Lichang,Zhou, Yan,Liu, Runxia,Li, Yanhua,Gao, Fei,Wang, Xiaomin,Yuan, Shishan,Wei, Zuzhang,Tong, Guangzhi,Fan, Hongjie,Wei, Zuzhang.

[14]Exchange of the VP5 of infectious bursal disease virus in a serotype I strain with that of a serotype II strain reduced the viral replication and cytotoxicity. Qin, Liting,Qi, Xiaole,Gao, Honglei,Gao, Yulong,Bu, Zhigao,Wang, Xiaomei. 2009

[15]Structural and biochemical insights into the V/I505T mutation found in the EIAV gp45 vaccine strain. Du, Jiansen,Ma, Jing,Wang, Jianxin,Liu, Fang,Qiao, Wentao,Liu, Xinqi,Wang, Xuefeng,Qin, Yuyin,Zhu, Chunhui,Zhou, Jianhua,Shao, Yiming,Shao, Yiming. 2014

[16]Autophagy activated by duck enteritis virus infection positively affects its replication. Yin, Hai-Chang,Zhao, Li-Li,Niu, Yin-Jie,Lu, Tao-Feng,Han, Ling-Xia,Liu, Sheng-Wang,Chen, Hong-Yan,Li, Si-Qi,Jiang, Xin-Jie,Xu, Li-Jing.

[17]Two amino acid residues in ion channel protein M2 and polymerase protein PA contribute to replication difference of H5N1 influenza viruses in mice. Xu, Chuantian,Hu, Beixia,Yang, Shaohua,Zhang, Lin,Huang, Qinghua,Zhang, Xiumei,Chen, Hualan,Chen, Hualan.

[18]PI3K/Akt/p53 pathway inhibits reovirus infection. Zhang, Xiaozhan,Wu, Hongxia,Liu, Chunguo,Tian, Jin,Qu, Liandong.

[19]Comparative study of the replication of infectious bursal disease virus in DF-1 cell line and chicken embryo fibroblasts evaluated by a new real-time RT-PCR. Wang, Yongqiang,Qi, Xiaole,Gao, Honglei,Gao, Yulong,Lin, Huan,Song, Xiuqing,Pei, Lei,Wang, Xiaomei.

[20]Properties and dissemination of H5N1 viruses isolated during an influenza outbreak in migratory waterfowl in western China. Chen, HL,Li, YB,Li, ZJ,Shi, JZ,Shinya, K,Deng, GH,Qi, QL,Tian, GB,Fan, SF,Zhao, HD,Sun, YX,Kawaoka, Y.

作者其他论文 更多>>