数字农科院2.0

Characteristics of molecular changes in lespedeza plants after seeds been flown on satellite

文献类型： 外文期刊

作者： Ren Weibo;Xu Zhu;Wang Zhaolan;Deng Bo;Han JianGuo;Zhang YunWei

作者机构：

关键词： Lespedeza bicolor;spaceflight;RAPD

期刊名称： AFRICAN JOURNAL OF BIOTECHNOLOGY

ISSN： 1684-5315

年卷期： 2009 年 8 卷 18 期

页码：

收录情况： SCI

摘要： To investigate the effect of space flight factors on lespedeza seeds, dry seeds of Lespedeza bicolor were loaded into recoverable satellite for space flight in 2003. After the retrieval, key agronomic traits were tested. Among the 65 plants we tested in the first generation (SP1), ten plants showed changed phenotype characters as prematurity, late-maturity, tall, dwarf, multi-ramify and etiolation. The mutation rate varied from 1.5 to 4.6% among different traits. 60 primers were screened in RAPD analysis to evaluate DNA variation between mutations and their ground controls. Results showed that 272 amplified bands produced by 55 primers. Among them, 12 primers amplified 41 polymorphic bands. Pattern of bands showed that absence of common bands were observed in mutated plants compared with the ground control. Moreover, similar band pattern was observed in the same mutation phenotype. Conclusion was that space flight factors could induce inheritable mutagenic changes on bush clover seeds, and verified these changes in genetic material in the mutants. These mutations may due to the breakage of chromosome fragments which was induced by complex traits of space flight, especially the cosmic radiation and microgravity. More work is being conducted to select the stable mutants with favorable traits and used them in developing new cultivars.

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