数字农科院2.0

Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.)

文献类型： 外文期刊

作者： Shi YongFeng ; Chen Jie ; Liu WenQiang ; Huang QiNa ; Shen Bo ; LEUNG Hei ; WU Jian Li *

作者机构：

关键词： rice (Oryza sativa L.);chemical mutagenesis;molecular marker;rolled-leaf gene;physical map

期刊名称： Science in China Series C: Life Sciences

ISSN： 1006-9305

年卷期： 2009 年 52 卷 9 期

页码：

收录情况： SCI

摘要： To understand the development of rice leaf blades, we identified a new rolled-leaf mutant, w32, from indica cultivar IR64 through EMS mutagenesis. The mutant showed a stable rolled-leaf phenotype throughout the life cycle. Two F-2 populations were developed by crossing w32 to cultivar IR24 and PA64. Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene. To determine the location of the gene, bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F-2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7. The rolled-leaf gene, tentatively named rl11(t), is likely a new gene as no other rolled-leaf genes have been identified near the region. By developing new SSR and InDel markers, the gene was delimited to a 52 kb region near the end of the short chromosome arm. Further fine mapping and cloning of the gene are currently underway.

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